Expression, crystallization and preliminary crystallographic data analysis of filamin A repeats 14-16
2007 (English)In: Acta Crystallographica. Section F: Structural Biology and Crystallization Communications, ISSN 1744-3091, E-ISSN 1744-3091, Vol. 63, no 4, 291-293 p.Article in journal (Refereed) Published
Human filamin A is a 280 kDa protein involved in actin-filament cross-linking. It is structurally divided into an actin-binding headpiece (ABD) and a rod domain containing 24 immunoglobulin-like (Ig) repeats. A fragment of human filamin A (Ig repeats 14-16) was cloned and expressed in Escherichia coli and the purified protein was crystallized in 1.6 M ammonium sulfate, 2% PEG 1000 and 100 mM HEPES pH 7.5. The crystals diffracted to 1.95 A and belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 50.63, b = 52.10, c = 98.46 A, alpha = beta = gamma = 90 degrees.
Place, publisher, year, edition, pages
2007. Vol. 63, no 4, 291-293 p.
ABP-280, Calponin homology, Cytoskeleton, Filamin
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-12674DOI: 10.1107/S1744309107006689ISI: 000245505800009PubMedID: 17401197OAI: oai:DiVA.org:uu-12674DiVA: diva2:40443