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The effects of handling time, ambient light, and anaesthetic method, on the standardized measurement of fish colouration
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Animal Ecology.
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2011 (English)In: Canadian Journal of Fisheries and Aquatic Sciences, ISSN 0706-652X, E-ISSN 1205-7533, Vol. 68, no 2, 330-342 p.Article in journal (Refereed) Published
Abstract [en]

The effects of handling time, ambient light intensity, and anaesthetic method, on the collection of spectral reflectance data were assessed in two species of Malawi cichlids (Melanochromis auratus and Metriaclima zebra). Using spectrometry, colour patches were measured over 10 min, under increasing ambient light levels, and using three anaesthetic methods. As time elapsed, maximum percent reflectance (R-max) decreased across all anaesthetic methods and species, suggesting that measurements should be taken immediately after capture and anaesthetization. With increasing light intensity, R-max increased significantly, suggesting that measurements should be taken under natural ambient conditions when possible. Finally, we found that anaesthetizing fish using an ice bath produced significantly higher R-max than using either MS-222 (tricaine methanesulphonate) or clove oil ( eugenol). However, the highest proportion of ultraviolet (UV) colouration was recorded while fish were anaesthetized with clove oil. Our results highlight the variation involved in measuring fish colour patterns using two related species, and thus indicate the need for a standardized approach to collecting spectral reflectance data in fish.

Place, publisher, year, edition, pages
2011. Vol. 68, no 2, 330-342 p.
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:uu:diva-149780DOI: 10.1139/F10-151ISI: 000288098700013OAI: oai:DiVA.org:uu-149780DiVA: diva2:405663
Available from: 2011-03-23 Created: 2011-03-23 Last updated: 2017-12-11Bibliographically approved

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