uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Degraded collagenase deteriorates islet viability
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Oncology, Radiology and Clinical Immunology, Clinical Immunology.
Show others and affiliations
2008 (English)In: Transplantation Proceedings, ISSN 0041-1345, E-ISSN 1873-2623, Vol. 40, no 2, 370-371 p.Article in journal (Refereed) Published
Abstract [en]

Objective. The utilization of purified enzyme blends consisting of collagenase class I (CI) and II (CII) and neutral protease is an essential step for clinical islet isolation. Previous studies suggested that the use of enzyme lots containing degraded CI reduced islet release from human pancreata. The present study sought to assess the effect of degraded collagenase on islet function in vitro and posttransplantation. Materials and Methods. Crude collagenase was chromatographically separated into CI, CII, and a mixture of degraded CI and CII isomers. Subsequently, classes were recombined to obtain a CII/CI ratio of 0.5. Rat islets were isolated utilizing neutral protease and 20 units of recombined collagenase containing either intact (Ci) or degraded isomers (Cd). Results. Digestion time was reduced utilizing Cd (P < .001). The highest islet yield and lowest islet fragmentation were obtained with Ci (P < .01). Utilization of Cd corresponded to a reduction in viability and in vitro function (NS). Islet transplantation reversed hyperglycemia in diabetic nude mice, but revealed an absence of weight gain in recipients receiving islets isolated using Cd (P < .01). Conclusion. This study suggested that islet function posttransplantation is affected by degraded collagenase isomers. This finding has to be considered for the purification process of collagenase.

Place, publisher, year, edition, pages
2008. Vol. 40, no 2, 370-371 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-149850DOI: 10.1016/j.transproceed.2008.01.013ISI: 000254695600012OAI: oai:DiVA.org:uu-149850DiVA: diva2:405910
Available from: 2011-03-24 Created: 2011-03-24 Last updated: 2011-03-24Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text
By organisation
Clinical Immunology
In the same journal
Transplantation Proceedings
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 138 hits
ReferencesLink to record
Permanent link

Direct link