uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Macrovascular changes in mice overexpressing human semicarbazide-sensitive amine oxidase in smooth muscle cells
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Pharmacology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Show others and affiliations
2007 (English)In: American Journal of Hypertension, ISSN 0895-7061, E-ISSN 1879-1905, Vol. 20, no 7, 743-750 p.Article in journal (Refereed) Published
Abstract [en]

Background: The catalytic activity of semicarbazide-sensitive amine oxidase (SSAO) is increased in diabetes, as well as in other disorders of cardiovascular origin. Our hypothesis is that SSAO is involved in the synthesis or maturation of elastin in vascular tissue. An increased SSAO activity can thereby be involved in the development of vascular damage. Methods: Elastin quantification was performed in aorta of transgenic mice overexpressing the human form of SSAO, using electron microscopy. Furthermore, lung capacity was measured using a spirometry-mimicking method, developed for mice. The effect of vasoactive substances was estimated by measuring mean arterial pressure and pulse pressure under anesthesia. Results: No differences in elastin quantity or lung capacity could be observed between transgenic or nontransgenic littermates. Pulse pressure was higher in transgenic mice, and electron microscopy of aorta showed elastin fibers parallel with the aorta wall (ie, straight fibers instead of folded compared with control mice). No difference in the response to adrenaline or sodium chloride was observed between the transgenic and control mice. The control mice had a clear decrease in blood pressure (BP) with a longer duration as a response to injection of a nitric oxide (NO) donor, sodium nitroprusside, compared with transgenic mice where only a minor response was observed. The SSAO activity in serum of control mice was elevated in response to injection of the NO donor, but not in response to a ganglion blocker. Conclusions: An elevated pulse pressure, together with an abnormal elastin structure in the aorta, suggests a rigidity of large arteries as a result of an elevated SSAO activity as well as a physiologic role for SSAO in elastin maturation.

Place, publisher, year, edition, pages
2007. Vol. 20, no 7, 743-750 p.
Keyword [en]
Amine oxidase, circulation, elastogenesis, pulse pressure, vascular complications
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-12858DOI: 10.1016/j.amjhyper.2007.02.011ISI: 000247979900006PubMedID: 17586408OAI: oai:DiVA.org:uu-12858DiVA: diva2:40628
Available from: 2008-03-05 Created: 2008-03-05 Last updated: 2014-10-03Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Forsberg-Nilsson, Karin
By organisation
PharmacologyDepartment of Medical Cell BiologyDepartment of Medical Biochemistry and Microbiology
In the same journal
American Journal of Hypertension
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 315 hits
ReferencesLink to record
Permanent link

Direct link