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Major ampullate spidroins from Euprosthenops australis: multiplicity at protein, mRNA and gene levels
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, The Linnaeus Centre for Bioinformatics.
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2007 (English)In: Insect molecular biology (Print), ISSN 0962-1075, E-ISSN 1365-2583, Vol. 16, no 5, 551-561 p.Article in journal (Refereed) Published
Abstract [en]

Spider dragline silk possesses extraordinary mechanical properties. It consists of large fibrous proteins called spidroins that display modular structures. It is known to consist of two proteins: the major ampullate spidroin (MaSp) 1 and MaSp2. This study analyses MaSp sequences from the nursery-web spider Euprosthenops australis. We have identified a previously uncharacterized MaSp2 sequence and a new MaSp-like spidroin, which display distinct homogenous submotifs within their respective Gly-rich repeats. Furthermore, a group of MaSp1 cDNA clones show unexpected heterogeneity. Genomic PCR identified several MaSp1 gene variants within individual spiders, which suggests the presence of a gene cluster in E. australis. Finally, the evolution of spidroin genes is discussed in relation to phylogenetic analysis of nonrepetitive C-terminal domains from diverse species.

Place, publisher, year, edition, pages
2007. Vol. 16, no 5, 551-561 p.
Keyword [en]
major ampullate spidroin, MaSp, spider, silk, evolution
National Category
Biological Sciences
URN: urn:nbn:se:uu:diva-150064DOI: 10.1111/j.1365-2583.2007.00749.xISI: 000249750600004PubMedID: 17680798OAI: oai:DiVA.org:uu-150064DiVA: diva2:406418
Available from: 2011-03-25 Created: 2011-03-25 Last updated: 2011-03-25Bibliographically approved

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