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Study of the non-covalent interaction between amyloid-beta-peptide and melatonin using electrospray ionization mass spectrometry
GAIA Research Center, The Goulandris Natural History Museum and Department of Pharmacy, Laboratory of Instrumental Analysis, University of Patras, Greece.
GAIA Research Center, The Goulandris Natural History Museum and Department of Pharmacy, Laboratory of Instrumental Analysis, University of Patras, Greece.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Analytical Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Analytical Chemistry.
2005 (English)In: Journal of Mass Spectrometry, ISSN 1076-5174, E-ISSN 1096-9888, Vol. 40, no 2, 182-192 p.Article in journal (Refereed) Published
Abstract [en]

Oxidative stress and unregulated immune response are believed to play a key role in the processes inherent to Alzheimer's disease (AD). The fact that free radicals can result in neurodegeneration suggests that actions against reactive oxygen species may be beneficial in treating and preventing AD. In the light of the suggested link between oxidative stress and AD, it is proposed that antioxidants and, even more, endogenous antioxidants may offer a therapeutic regime for protection against the risk of this disease. For this reason, the formation of non-covalent complexes between amyloid-beta-peptide (A beta) or its oxidized forms and melatonin was studied by quadrupole and Fourier transform ion cyclotron resonance electrospray ionization mass spectrometry. The stability of the non-covalent complex was examined under several experimental conditions, such as orifice voltage, pH, presence of organic modifier, concentration and time. Two different digestion protocols combined with mass spectrometric analysis of the resulting peptide fragments were employed in order to locate the binding site of melatonin in A beta.

Place, publisher, year, edition, pages
2005. Vol. 40, no 2, 182-192 p.
Keyword [en]
Alzheimer's disease, amyloid-beta-peptide, melatonin, non-covalent complexes, electrospray ionization mass spectrometry
National Category
Chemical Sciences Pharmaceutical Sciences
Identifiers
URN: urn:nbn:se:uu:diva-150437DOI: 10.1002/jms.738PubMedID: 15706607OAI: oai:DiVA.org:uu-150437DiVA: diva2:407372
Available from: 2011-03-30 Created: 2011-03-30 Last updated: 2017-12-11Bibliographically approved

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Markides, KarinBergquist, Jonas

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