uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Mifepristone, but not levonorgestrel, inhibits human blastocyst attachment to an in vitro endometrial three-dimensional cell culture model
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Women's and Children's Health. (Klinisk och experimentell reproduktionsbiologi/Olovsson)
Show others and affiliations
2007 (English)In: Human Reproduction, ISSN 0268-1161, E-ISSN 1460-2350, Vol. 22, no 11, 3031-3037 p.Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: The use of fertility regulating drugs is limited among various socio-ethnic groups due to limited knowledge about their mechanism of action. This study investigates the effect of levonorgestrel and mifepristone on attachment of human embryos to an in vitro endometrial construct. METHOD: Three-dimensional endometrial constructs were established by co-culturing early luteal phase human endometrial stromal and epithelial cells. Expression of endometrial receptivity markers in this construct were examined by immunohistochemistry. Effects of mifepristone and levonorgestrel on viability and attachment of human blastocysts were investigated. RESULTS: Endometrial constructs expressed the factors involved in endometrial receptivity: estrogen receptor, progesterone receptor, vascular endothelial growth factor, leukemia inhibitory factor, interleukin-1, COX-2, MUC-1 and integrin-alpha(V)beta(3). None of the 15 embryos cultured with mifepristone attached to the endometrial construct (P < 0.01), whereas 10/17 in control, and 6/14 in levonorgestrel, groups attached. The attachment was confirmed by the positive expression of cytokeratin 7 at the attachment site. CONCLUSION: Mifepristone inhibits blastocyst attachment. Levonorgestrel did not impair the attachment of human embryos to the in vitro endometrial construct. This model could be used to understand endometrial receptivity and embryo-endometrial dialog and to develop new fertility regulating substances.

Place, publisher, year, edition, pages
2007. Vol. 22, no 11, 3031-3037 p.
Keyword [en]
3D-endometrial cell construct, receptivity markers, mifepristone, levonorgestrel, human blastocyst
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-13044DOI: 10.1093/humrep/dem297ISI: 000251371400033PubMedID: 17890724OAI: oai:DiVA.org:uu-13044DiVA: diva2:40814
Available from: 2008-02-19 Created: 2008-02-19 Last updated: 2010-03-25Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Stavreus-Evers, A
By organisation
Department of Women's and Children's Health
In the same journal
Human Reproduction
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 122 hits
ReferencesLink to record
Permanent link

Direct link