uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Horseradish peroxidase dye tracing and embryonic statoacoustic ganglion cell transplantation in the rat auditory nerve trunk
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Physiology.
Show others and affiliations
2011 (English)In: Brain Research, ISSN 0006-8993, E-ISSN 1872-6240, Vol. 1377, 41-49 p.Article in journal (Refereed) Published
Abstract [en]

At present severe damage to hair cells and sensory neurons in the inner ear results in non-treatable auditory disorders. Cell implantation is a potential treatment for various neurological disorders and has already been used in clinical practice. In the inner ear, delivery of therapeutic substances including neurotrophic factors and stem cells provide strategies that in the future may ameliorate or restore hearing impairment. In order to describe a surgical auditory nerve trunk approach, in the present paper we injected the neuronal tracer horseradish peroxidase (HRP) into the central part of the nerve by an intra cranial approach. We further evaluated the applicability of the present approach by implanting statoacoustic ganglion (SAG) cells into the same location of the auditory nerve in normal hearing rats or animals deafened by application of p-bungarotoxin to the round window niche. The HRP results illustrate labeling in the cochlear nucleus in the brain stem as well as peripherally in the spiral ganglion neurons in the cochlea. The transplanted SAGs were observed within the auditory nerve trunk but no more peripheral than the CNS-PNS transitional zone. Interestingly, the auditory nerve injection did not impair auditory function, as evidenced by the auditory brainstem response. The present findings illustrate that an auditory nerve trunk approach may well access the entire auditory nerve and does not compromise auditory function. We suggest that such an approach might compose a suitable route for cell transplantation into this sensory cranial nerve.

Place, publisher, year, edition, pages
2011. Vol. 1377, 41-49 p.
Keyword [en]
Auditory nerve, Horseradish peroxidase, Statoacoustic ganglion, Cell transplantation, Transitional zone
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-150680DOI: 10.1016/j.brainres.2010.12.078ISI: 000288299900004PubMedID: 21215730OAI: oai:DiVA.org:uu-150680DiVA: diva2:408249
Available from: 2011-04-04 Created: 2011-04-04 Last updated: 2011-04-04Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed
By organisation
In the same journal
Brain Research
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 153 hits
ReferencesLink to record
Permanent link

Direct link