Shb null allele is inherited with a transmission ratio distortion and causes reduced viability in utero
2007 (English)In: Developmental Dynamics, ISSN 1058-8388, E-ISSN 1097-0177, Vol. 236, no 9, 2485-2492 p.Article in journal (Refereed) Published
SHB is an Src homology 2 domain-containing adapter protein that has been found to be involved in numerous cellular responses. We have generated an Shb knockout mouse. No Shb-/- pups or embryos were obtained on the C57Bl6 background, indicating an early defect as a consequence of Shb- gene inactivation on this genetic background. Breeding heterozygotes for Shb gene inactivation (Shb+/-) on a mixed genetic background (FVB/C57Bl6/129Sv) reveals a distorted transmission ratio of the null allele with reduced numbers of Shb+/+ and Shb-/- animals, but increased number of Shb+/- animals. The Shb- allele is associated with various forms of malformations, explaining the relative reduction in the number of Shb-/- offspring. Shb-/- animals that were born were viable, fertile, and showed no obvious defects. However, Shb+/- female mice ovulated preferentially Shb- oocytes explaining the reduced frequency of Shb+/+ mice. Our study suggests a role of SHB during reproduction and development.
Place, publisher, year, edition, pages
2007. Vol. 236, no 9, 2485-2492 p.
Knockout, Malformations, Ovulation, SHB, Transmission ratio distortion, Viability in utero
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-13084DOI: 10.1002/dvdy.21257ISI: 000249539700012PubMedID: 17676633OAI: oai:DiVA.org:uu-13084DiVA: diva2:40854