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Immobilization of oligonucleotide-functionalized magnetic nanobeads in DNA-coils studied by electron microscopy and atomic force microscopy
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences. (Electron Microscopy and Nanoengineering)
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Solid State Physics.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.
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2011 (English)In: MRS Online Proceedings Library: volume 1355 / [ed] L. Stanciu, S. Andreescu, T. Noguer, B. Liu, 2011Conference paper, Published paper (Refereed)
Abstract [en]

Immobilization of oligonucleotide-functionalized magnetic nanobeads by hybridization in DNA-coils formed by rolling circle amplification has been investigated using transmission electron microscopy (TEM) and atomic force microscopy (AFM). The TEM results supported earlier made observations that small beads with low oligonucleotide surface coverage preferably immobilize in the interior of the DNA-coils and do not tend to link several DNA-coils together whereas large beads with high surface coverage to a larger extent connect several DNA-coils together to clusters of several DNA-coils with beads. AFM provided direct visualization of the DNA-coils as thread-like objects. DNA-coils with immobilized beads appeared as a collection of beads with thread-like features in their near vicinity.

Place, publisher, year, edition, pages
2011.
Keyword [en]
nanoscale; transmission electron microscopy (TEM); colloid
National Category
Other Materials Engineering
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials; Engineering Science with specialization in Solid State Physics; Engineering Science with specialization in Materials Science
Identifiers
URN: urn:nbn:se:uu:diva-150876DOI: 10.1557/opl.2011.1135OAI: oai:DiVA.org:uu-150876DiVA: diva2:409115
Conference
MRS Spring Meeting 2011, Symposium JJ – Biological Hybrid Materials for Life Sciences, April 26-29, 2011, San Francisco, California, USA
Available from: 2011-04-07 Created: 2011-04-07 Last updated: 2016-12-01Bibliographically approved

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Publisher's full texthttp://www.mrs.org/spring2011/

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Strömberg, MattiasAkhtar, SultanGunnarsson, KlasZardán Gómez de la Torre, TeresaRussell, CamillaHerthnek, DavidSvedlindh, PeterNilsson, MatsStrømme, MariaLeifer, Klaus

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Strömberg, MattiasAkhtar, SultanGunnarsson, KlasZardán Gómez de la Torre, TeresaRussell, CamillaHerthnek, DavidSvedlindh, PeterNilsson, MatsStrømme, MariaLeifer, Klaus
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Nanotechnology and Functional MaterialsApplied Materials SciencesSolid State PhysicsMolecular tools
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