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Comparing the tube and gel techniques for ABO antibody titration, as performed in three European centers
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Oncology, Radiology and Clinical Immunology.
2007 (English)In: Transplantation, ISSN 0041-1337, E-ISSN 1534-6080, Vol. 84, no 12, S17-S19 p.Article in journal (Refereed) Published
Abstract [en]

Data from 60 consecutive ABO-incompatible kidney transplantations performed in Stockholm, Sweden; Freiburg, Germany; and Uppsala, Sweden, revealed significant variation in preoperative A/B antibody levels, with median titers of 1:32, 1:128, and 1:8, respectively. We wanted to investigate whether these differences were method-related. The same samples from 21 healthy blood donors were analyzed in the three centers using current local methods. Results confirmed method-related differences, with higher A/B titers in Freiburg and lower titers in Uppsala compared with Stockholm. Results for the same sample differed by a median of three (range 0 to 6) titer steps. When the same number of samples were analyzed in the three centers using the same gel method and the same test erythrocytes, results differed by a median of one titer step (range 0 to 4) for the same sample. In conclusion, gel hemagglutination technique significantly decreases intercenter variation compared with tube technique.

Place, publisher, year, edition, pages
2007. Vol. 84, no 12, S17-S19 p.
Keyword [en]
A/B antibody, A/B antibody titration, ABO antibody, ABO antibody titration, direct agglutination, immunoglobulin G (IgG), immunoglobulin M (IgM), indirect agglutination
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-150961DOI: 10.1097/01.tp.0000296019.85986.afISI: 000252146600007PubMedID: 18162980OAI: oai:DiVA.org:uu-150961DiVA: diva2:409504
Available from: 2011-04-08 Created: 2011-04-08 Last updated: 2017-12-11Bibliographically approved

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