Comparing the tube and gel techniques for ABO antibody titration, as performed in three European centers
2007 (English)In: Transplantation, ISSN 0041-1337, E-ISSN 1534-6080, Vol. 84, no 12, S17-S19 p.Article in journal (Refereed) Published
Data from 60 consecutive ABO-incompatible kidney transplantations performed in Stockholm, Sweden; Freiburg, Germany; and Uppsala, Sweden, revealed significant variation in preoperative A/B antibody levels, with median titers of 1:32, 1:128, and 1:8, respectively. We wanted to investigate whether these differences were method-related. The same samples from 21 healthy blood donors were analyzed in the three centers using current local methods. Results confirmed method-related differences, with higher A/B titers in Freiburg and lower titers in Uppsala compared with Stockholm. Results for the same sample differed by a median of three (range 0 to 6) titer steps. When the same number of samples were analyzed in the three centers using the same gel method and the same test erythrocytes, results differed by a median of one titer step (range 0 to 4) for the same sample. In conclusion, gel hemagglutination technique significantly decreases intercenter variation compared with tube technique.
Place, publisher, year, edition, pages
2007. Vol. 84, no 12, S17-S19 p.
A/B antibody, A/B antibody titration, ABO antibody, ABO antibody titration, direct agglutination, immunoglobulin G (IgG), immunoglobulin M (IgM), indirect agglutination
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-150961DOI: 10.1097/01.tp.0000296019.85986.afISI: 000252146600007PubMedID: 18162980OAI: oai:DiVA.org:uu-150961DiVA: diva2:409504