uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Elongation factor 1-alpha is released into the culture medium during growth of Giardia intestinalis trophozoites
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm , Ludwig Institute for Cancer Research.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Microbiology.
2011 (English)In: Experimental parasitology, ISSN 0014-4894, E-ISSN 1090-2449, Vol. 127, no 4, 804-810 p.Article in journal (Refereed) Published
Abstract [en]

The molecular pathogenesis of the intestinal parasite Giardia intestinalis is still not fully understood but excretory-secretory products have been suggested to be important during host-parasite interactions. Here we used SOS-PAGE gels and MALDI-TOF analysis to identify proteins released by Giardia trophozoites during in vitro growth. Serum proteins (mainly bovine serum albumin) in the growth medium, bind to the parasite surface and they are continuously released, which interfere with parasite secretome characterization. However, we identified two released Giardia proteins: elongation factor-1 alpha (EF-1 alpha) and a 58 kDa protein, identified as arginine deiminase (ADI). This is the first description of EF-1 alpha as a released/secreted Giardia protein, whereas ADI has been identified in an earlier secretome study. Two genes encoding EF-1 alpha were detected in the Giardia WB genome 35 kbp apart with almost identical coding sequences but with different promoter and 3' regions. Promoter luciferase-fusions showed that both genes are transcribed in trophozoites. The EF-1 alpha protein localizes to the nuclear region in trophozoites but it relocalizes to the cytoplasm during host-cell interaction. Recombinant EF-1 alpha is recognized by serum from giardiasis patients. Our results suggest that released EF-1 alpha protein can be important during Giardia infections.

Place, publisher, year, edition, pages
2011. Vol. 127, no 4, 804-810 p.
Keyword [en]
Giardia, Elongation factor 1 alpha, Secretory-excretory products, Intestinal parasite, Virulence factor
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:uu:diva-151758DOI: 10.1016/j.exppara.2011.01.006ISI: 000288592400012PubMedID: 21276445OAI: oai:DiVA.org:uu-151758DiVA: diva2:411296
Available from: 2011-04-18 Created: 2011-04-18 Last updated: 2011-04-18Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed
By organisation
Department of Cell and Molecular BiologyLudwig Institute for Cancer ResearchMicrobiology
In the same journal
Experimental parasitology
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 349 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf