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In vivo Extravasated Human Monocytes have an Altered Expression of CD16, HLA-DR, CD86, CD36 and CX(3)CR1
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm, UCR-Uppsala Clinical Research Center. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences.
2009 (English)In: Scandinavian Journal of Immunology, ISSN 0300-9475, E-ISSN 1365-3083, Vol. 70, no 4, 368-376 p.Article in journal (Refereed) Published
Abstract [en]

The phenotypic alterations in monocytes induced by extravasation in vivo are still largely unknown. We addressed the question whether a general phenotype of extravasated monocytes exists and whether this phenotype differs between healthy individuals and statin treated patients with coronary artery disease (CAD). In vivo extravasated monocytes from CAD patients and healthy controls were collected by use of the skin blister method and compared with peripheral circulating monocytes by flow cytometry. The number of CD14(+)CD16(+) monocytes were significantly higher in the skin blister compared with peripheral circulation in both patients (P < 0.001) and controls (P = 0.005). In vivo extravasated monocytes had in comparison with peripheral monocytes a lower expression Of CX(3)CR1, a higher expression of HLA-DR, CD86 and CD36 and a higher binding of acetylated low density lipoprotein (acLDL) (significant for all markers). Skin blister fluid from CAD patients, compared with healthy controls, induced a 206 increase in monocyte CD36 expression (P = 0.008) following 18 h of in vitro incubation The results indicate that the integrated. response to the in vivo extravasation process is similar in statin treated stable CAD patients and healthy controls, with respect to phenotypic alterations. Such differences in CAD patients may, however, occur as a response to the inflammatory milieu.

Place, publisher, year, edition, pages
2009. Vol. 70, no 4, 368-376 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-152104DOI: 10.1111/j.1365-3083.2009.02306.xISI: 000270227000006OAI: oai:DiVA.org:uu-152104DiVA: diva2:412494
Available from: 2011-04-25 Created: 2011-04-25 Last updated: 2014-02-21Bibliographically approved

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Held, Claes
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UCR-Uppsala Clinical Research CenterDepartment of Medical Sciences
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