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Diverse splicing pathways of the membrane IgHM pre-mRNA in a Chondrostean, the Siberian sturgeon
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
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2009 (English)In: Developmental and Comparative Immunology, ISSN 0145-305X, E-ISSN 1879-0089, Vol. 33, no 4, 507-515 p.Article in journal (Refereed) Published
Abstract [en]

Teleosts and tetrapods have evolved different splice patterns to generate their membrane-bound IgM. In the tetrapod lineage, the first transmembrane exon is spliced to an internal cryptic site located close to the end of the fourth constant exon. Because teleosts lack this site they use the regular T-splice site of the CH3 exon instead. We characterized the mu m splicing patterns in a Chondrostean, the Siberian sturgeon. We observed a surprising diversity of splice patterns, the TM1 exon being spliced to a cryptic site at the end of CH4, to a cryptic site in CH3 or to the 3'-end of CHI. These different pathways lead to m1GHM transcripts encoding four, two or one complete C-domain(s), respectively. The short variant CH1-TM1 was found only in VH2 positive transcripts, while the two other variants were observed for IgHM transcripts expressing all VH families. These results shed light on the evolution of IgM splicing pathways.

Place, publisher, year, edition, pages
2009. Vol. 33, no 4, 507-515 p.
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Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-152120DOI: 10.1016/j.dci.2008.10.009ISI: 000263781100011OAI: oai:DiVA.org:uu-152120DiVA: diva2:412510
Available from: 2011-04-25 Created: 2011-04-25 Last updated: 2017-12-11Bibliographically approved

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