Genomic sequencing and genealogical analysis of DNA from a prehistoric dog
Independent thesis Advanced level (degree of Master (Two Years)), 30 credits / 45 HE creditsStudent thesis
Next generation sequencing technology stands as one of the most promising advances in the field of paleogenetics. In this study, high-throughput shotgun sequencing of amplified metagenomic libraries was used to generate low-coverage genome data from an approximately 5000 year old dog specimen from Ajvide (Gotland, Sweden). I show that a genealogical approach can be used to estimate the split time between an ancient population and two current populations for which medium- to high-coverage genome assemblies are available, without the need for assumptions on mutation rate. Applied to the presented data, the analysis indicates that there was extensive structure between the Neolithic Scandinavian dog population and the ancestors of modern boxers and poodles. This observation is best explained either by an early Upper Paleolithic origin of domestic dogs, multiple domestication events, or extensive backcrossing with their lupine progenitors. The study shows that as reference genomic data from extant populations accumulate, multilocus data generated from a single well-preserved specimen analyzed using this approach can provide powerful insights into prehistoric demography and evolutionary history.
Place, publisher, year, edition, pages
2009. , 54 p.
IdentifiersURN: urn:nbn:se:uu:diva-152165OAI: oai:DiVA.org:uu-152165DiVA: diva2:412734
Master Programme in Biology
2009-06-03, 14:30 (English)
UppsokLife Earth Science
Götherström, Anders, Dr.