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Epitope mapping of human aromatic L-amino acid decarboxylase
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences. (Autoimmuna sjukdomar (Kämpe))
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2007 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 353, no 3, 692-698 p.Article in journal (Refereed) Published
Abstract [en]

Autoimmune polyendocrine syndrome type I (APS I) is a rare hereditary condition considered a model disease for organ specific autoimmunity. A wide range of autoantibodies targeting antigens present in the affected organs have been identified. Autoantibodies against aromatic l-amino acid decarboxylase (AADC) are present in about 50% of APS I patients. In order to increase our understanding of autoantibody specificity in APS I, the aim of the present study was to localize target regions on AADC recognized by sera from APS I patients. Using several complementing strategies, we have shown that autoantibodies against AADC mainly recognize conformational epitopes. The major antigenic determinants were detected N-terminally to amino acid residue 237. Replacement of amino acids 227–230 (ERDK) with alanine residues reduced the reactivity towards AADC by >80% in all patient sera tested, suggesting that amino acids 227–230 are an important part of an immunodominant epitope.

Place, publisher, year, edition, pages
2007. Vol. 353, no 3, 692-698 p.
Keyword [en]
Aromatic l-amino acid decarboxylase, Autoantibodies, Autoimmune polyendocrine syndrome type I, Epitope mapping
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-13710DOI: 10.1016/j.bbrc.2006.12.080ISI: 000243570000028PubMedID: 17194446OAI: oai:DiVA.org:uu-13710DiVA: diva2:41480
Available from: 2008-01-25 Created: 2008-01-25 Last updated: 2011-02-16Bibliographically approved

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