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Fresh frozen tissue: RNA extraction and quality control
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology. (Botling)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology. (Botling)ORCID iD: patmi676
2011 (English)In: Methods in Molecular Biology, ISSN 1064-3745, E-ISSN 1940-6029, Vol. 675, 405-413 p.Article in journal (Refereed) Published
Abstract [en]

Since RNA is believed to be the most vulnerable molecular component of unfixed tissue, preserved RNA integrity can be used as a general quality indicator in fresh frozen tissue biobanks. As the size of samples and biopsies often is small, in the range of millimeters or milligrams, it is important to implement quality control procedures adapted to minute the amounts of tissue. To this end, we here describe RNA extraction from one or a few frozen tissue sections and subsequent analysis of structural RNA integrity by microcapillary gel electrophoresis.

Place, publisher, year, edition, pages
2011. Vol. 675, 405-413 p.
Keyword [en]
Biobank, Fresh frozen tissue, RNA extraction, RNA quality, Bioanalyzer. RIN
National Category
Cell and Molecular Biology
Research subject
Pathology
Identifiers
URN: urn:nbn:se:uu:diva-153105DOI: 10.1007/978-1-59745-423-0_25PubMedID: 20949406OAI: oai:DiVA.org:uu-153105DiVA: diva2:415183
Available from: 2011-05-05 Created: 2011-05-05 Last updated: 2017-12-12Bibliographically approved

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Botling, JohanMicke, Patrick

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