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Biobanking of fresh frozen tissue from clinical surgical specimens: transport logistics, sample selection, and histologic characterization.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Clinical and experimental pathology. (Botling)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Experimental and Clinical Oncology. (Botling)ORCID iD: patmi676
2011 (English)In: Methods in Molecular Biology, ISSN 1064-3745, E-ISSN 1940-6029, Vol. 675, 299-306 p.Article in journal (Refereed) Published
Abstract [en]

Access to high-quality fresh frozen tissue is critical for translational cancer research and molecular -diagnostics. Here we describe a workflow for the collection of frozen solid tissue samples derived from fresh human patient specimens after surgery. The routines have been in operation at Uppsala University Hospital since 2001. We have integrated cryosection and histopathologic examination of each biobank sample into the biobank manual. In this way, even small, macroscopically ill-defined lesions can be -procured without a diagnostic hazard due to the removal of uncharacterized tissue from a clinical -specimen. Also, knowledge of the histomorphology of the frozen tissue sample - tumor cell content, stromal components, and presence of necrosis - is pivotal before entering a biobank case into costly molecular profiling studies.

Place, publisher, year, edition, pages
2011. Vol. 675, 299-306 p.
Keyword [en]
Biobank, Molecular pathology, Fresh frozen tissue, Cryosection, Quality control
National Category
Cell and Molecular Biology Cell and Molecular Biology
Research subject
Pathology
Identifiers
URN: urn:nbn:se:uu:diva-153106DOI: 10.1007/978-1-59745-423-0_16PubMedID: 20949397OAI: oai:DiVA.org:uu-153106DiVA: diva2:415185
Available from: 2011-05-05 Created: 2011-05-05 Last updated: 2017-12-12Bibliographically approved

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Botling, JohanMicke, Patrick

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