IL-10 is up regulated in early and transitional stages in vervet monkeys experimentally infected with Trypanosoma brueei rhodesiense
2006 (English)In: Parasitology international, ISSN 1383-5769, Vol. 55, no 4, 243-248 p.Article in journal (Refereed) Published
IL-10 has been suggested as a possible parameter for human African trypanosomiasis stage determination. However, conclusive experimental studies have not been carried out to evaluate this, which is a prerequisite before a potential test can be validated in humans for diagnostic purposes. We used the vervet monkey model of trypanosomiasis to scrutinize IL-10 in blood and cerebrospinal fluid (CSF). Five adult males were experimentally infected with T.b. rhodesiense. The infected animals became anemic and exhibited weight loss. Parasitemia was patent after 3 days and fluctuated around 3.7 x 10(7) trypanosomes/ml throughout the experimental period. The total CSF white cell counts increased from pre-infection means around 3 cells/mu l to a peak of 30 cells/mu l, 42 days post-infection (DPI). IL-10 was not detectable (< 2 pg/ml) in serum prior to infection. IL-10 serum concentrations increased to 273 pg/ml 10 DPI coinciding with the first peak of parasitemia. Thereafter the levels declined to a mean value of 77 pg/ml 34 DPI followed by a significant rise to a second peak of 304 pg/ml (p < 0.008) 42 DPI. There was no detectable IL-10 in CSF. IL-10 synthesis is thus stimulated both in the early and transitional stages of experimental trypanosomiasis. That IL-10 is produced in early stage disease is an interesting finding unlikely to be detected in humans where it is difficult to determine the exact time of infection. The IL-10 peak observed on day 42 of infection might indicate onset of parasite neuroinvasion coinciding with a peak in white blood cell counts in the blood and CSF.
Place, publisher, year, edition, pages
2006. Vol. 55, no 4, 243-248 p.
Trypanosoma brucei rhodesiense, Chlorocebus aethiops, Cereopithecus aethiops, vervet monkey, African green monkey, human African trypanosomiasis, sleeping sickness, IL-10, diagnosis
IdentifiersURN: urn:nbn:se:uu:diva-153160DOI: 10.1016/j.parint.2006.06.004ISI: 000242510100002PubMedID: 16901747OAI: oai:DiVA.org:uu-153160DiVA: diva2:415407