uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Biomedicine in the environment: Cyclotides constitute potent natural toxins in plants and soil bacteria
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Medicinal Chemistry, Division of Pharmacognosy. (Göransson)
Show others and affiliations
2011 (English)In: Environmental Toxicology and Chemistry, ISSN 0730-7268, E-ISSN 1552-8618, Vol. 30, no 5, 1190-1196 p.Article in journal (Refereed) Published
Abstract [en]

Bioactive compounds produced by plants are easily transferred to soil and water and may cause adverse ecosystem effects. Cyclotides are gene-encoded, circular, cystine-rich mini-proteins produced in Violaceae and Rubiaceae in high amounts. Based on their biological activity and stability, cyclotides have promising pharmaceutical and agricultural applications. We report the toxicity of the cyclotides: kalata B1, kalata B2, and cycloviolacin O2 extracted from plants to green algae (Pseudokirchneriella subcapitata), duckweed (Lemna minor L.), lettuce (Lactuca sativa L.), and bacteria extracted from soil measured as [3H]leucine incorporation. Quantification by liquid chromatography-mass spectrometry demonstrated up to 98% loss of cyclotides from aqueous solutions because of sorption to test vials. Sorption was prevented by adding bovine serum albumin (BSA) to the aqueous media. Cyclotides were toxic to all test organisms with EC50 values of 12 through 140 µM (algae), 9 through 40 µM (duckweed), 4 through 54 µM (lettuce), and 7 through 26 µM (bacteria). Cycloviolacin O2 was the most potent cyclotide in all assays examined. This report is the first to document toxic effects of cyclotides in plants and soil bacteria and to demonstrate that cyclotides are as toxic as commonly used herbicides and biocides. Hence, cyclotides may adversely affect soil and aquatic environments, which needs to be taken into account in future risk assessment of cropping systems for production of these highly bioactive compounds.

Place, publisher, year, edition, pages
2011. Vol. 30, no 5, 1190-1196 p.
Keyword [en]
Cyclotides, Ecotoxicity, Biomedicine, Natural toxins, True exposure concentration
National Category
Biological Sciences
URN: urn:nbn:se:uu:diva-153299DOI: 10.1002/etc.496ISI: 000289820400022PubMedID: 21337607OAI: oai:DiVA.org:uu-153299DiVA: diva2:416015
Available from: 2011-05-10 Created: 2011-05-10 Last updated: 2012-03-22Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Göransson, Ulf
By organisation
Division of Pharmacognosy
In the same journal
Environmental Toxicology and Chemistry
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 291 hits
ReferencesLink to record
Permanent link

Direct link