beta-Cyclodextrin grafted polyethyleneimine hydrogel immobilizing hydrophobically modified glucose oxidase
2011 (English)In: International Journal of Biological Macromolecules, ISSN 0141-8130, E-ISSN 1879-0003, Vol. 48, no 4, 661-666 p.Article in journal (Refereed) Published
Hydrogels which release their contents in response to glucose concentration were prepared by immobilizing glucose oxidase (GOD) into beta-cyclodextrin grafted polyethyleneimine hydrogels (PEI-beta CD hydrogel). For the tight immobilization, hydrophobically modified GOD (HmGOD) was prepared by reacting GOD with palmitic acid-N-hydroxysuccinimide ester (PA-NHS) in the molar ratio of 1:40. According to trinitrobenzene sulfonic acid (TNBS) assay, five palmitic acids were covalently attached to one GOD molecule. The activity of HmGOD was about 76% of native enzyme. The swelling ratios of HmGOD loaded hydrogels increased from about 960% to 1190% in 24 h, when glucose concentration was varied from 0 to 100 mg/dl. The % release in 48 h of fluorescein isothiocyanate dextran increased from about 53% to 89%, when glucose concentration was varied in the same range. Gluconic acid, produced by the enzymatic reaction, would protonate and swell the PEI-beta CD hydrogel, leading to a higher release.
Place, publisher, year, edition, pages
2011. Vol. 48, no 4, 661-666 p.
beta-Cyclodextrin, Polyethyleneimine, Hydrogels, Hydrophobic association, Glucose-sensitivity
Chemical Sciences Polymer Chemistry
Research subject Chemistry with specialization in Polymer Chemistry
IdentifiersURN: urn:nbn:se:uu:diva-153289DOI: 10.1016/j.ijbiomac.2011.02.007ISI: 000289745900019PubMedID: 21334372OAI: oai:DiVA.org:uu-153289DiVA: diva2:416045