Webtag: A new web tool providing tags/anchors for RT-PCR experiments with prokaryotes
2007 (English)In: BMC Biotechnology, ISSN 1472-6750, Vol. 7, 73- p.Article in journal (Refereed) Published
Background: Webtag is a tool providing oligonucleotide sequences (usually called tags or anchors) that are absent from a specified genome. These tags/anchors can be appended to gene specific primers for reverse transcriptase polymerase chain reaction experiments, circumventing genomic DNA contamination. Results: The use of a relational database, in conjunction with a series of scripts written in PHP and Perl, allows the user to rapidly obtain tags that are: 1) suitable for a specific organism, and 2) compatible with other oligonucleotides to be used in the experimental procedures. Conclusion: This new web tool allows scientists to easily and rapidly obtain suitable tags for RTPCR experiments, and is available at http://www.egs.uu.se/software/webtag/.
Place, publisher, year, edition, pages
2007. Vol. 7, 73- p.
IdentifiersURN: urn:nbn:se:uu:diva-13840DOI: 10.1186/1472-6750-7-73ISI: 000251903100001PubMedID: 17961214OAI: oai:DiVA.org:uu-13840DiVA: diva2:41610