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Lymphogranuloma venereum prevalence in Sweden among men who have sex with men and characterization of Chlamydia trachomatis ompA genotypes
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Bacteriology.
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2006 (English)In: Journal of Clinical Microbiology, ISSN 0095-1137, E-ISSN 1098-660X, Vol. 44, no 11, 4066-4071 p.Article in journal (Refereed) Published
Abstract [en]

An outbreak of lymphogranuloma venereum (LGV) infections has recently been reported from The Netherlands and other European countries. The Swedish surveillance system has identified three LGV cases since 2004, all with clinically suspected infection in men who have sex with men (MSM). In order to assess the prevalence of LGV in a high-risk group of MSM and include clinically atypical cases, retrospective analysis of 197 Chlamydia trachomatis-infected men was performed. Sequencing of the ompA gene showed a different serotype distribution compared to recent Swedish studies in heterosexual populations. The most common types were G (45%), D (27%), and J (26%), whereas the normally predominant type E accounted for only 4% of the chlamydia cases. Furthermore, certain ompA genotype variants of the dominant serotypes were highly prevalent among MSM, and the reason for this is discussed. No additional case of LGV was detected by retrospective analysis of the high-risk MSM population. This indicates that, thus far, LGV in Sweden is only a result of sporadic import from infected MSM clusters abroad.

Place, publisher, year, edition, pages
2006. Vol. 44, no 11, 4066-4071 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-13846DOI: 10.1128/JCM.00574-06ISI: 000242056800034PubMedID: 16971651OAI: oai:DiVA.org:uu-13846DiVA: diva2:41616
Available from: 2008-12-03 Created: 2008-12-03 Last updated: 2011-05-12Bibliographically approved
In thesis
1. Chlamydia trachomatis: Development of molecular typing methods and applications in epidemiology
Open this publication in new window or tab >>Chlamydia trachomatis: Development of molecular typing methods and applications in epidemiology
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

A general aim was to combine molecular typing methods with clinical background information to increase epidemiological knowledge about Chlamydia trachomatis infections.

An outbreak of Lymfogranuloma venereum (LGV), caused by a more invasive variant of C. trachomatis, was reported from the Netherlands in 2003 among men who have sex with men (MSM). All Chlamydia positive specimens from a venereal disease clinic for MSM in Stockholm during one year were genotyped. No spread of LGV was found, apart from three symptomatic cases. The same ompA genotypes were found among MSM in Melbourne, but the genotype distribution was different compared to findings among the heterosexual population in Sweden.

The standard method for genotyping of Chlamydia is ompA-sequencing, but it has low resolution because one genotype predominates. A multilocus sequence typing (MLST) system based on five targets was developed. In a sample of 47 specimens, 32 variants were found with MLST, but only 12 variants with ompA-sequencing. The polymorphisms in the hctB gene, one MLST target, are caused by an element of 108 bp that is present in two to four repetitions and in different variants. Although the DNA-binding function of Hc2 that is encoded by hctB has been studied, our findings of a considerable size variation show that new studies are needed.

In 2006, specimens with a 377 bp deletion in the cryptic plasmid covering the target region for diagnostic test systems from Abbott and Roche were discovered in Sweden. Applying MLST to these specimens indicated that there was a single clone, denoted nvCT. The proportion of nvCT in all detected Chlamydia cases was higher (20% to 65%) in counties using Abbott/Roche compared to counties using the BectonDickinson test system (7% to 20%). The proportions of nvCT converge in counties with high or low levels when detection systems were adjusted to detect nvCT.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2009. 71 p.
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 1651-6206 ; 488
Chlamydia trachomatis, Lymphogranuloma venereum, Genotyping, MLST, nvCT, hctB, Hc2
National Category
Microbiology in the medical area
Research subject
Clinical Bacteriology
urn:nbn:se:uu:diva-108930 (URN)978-91-554-7625-0 (ISBN)
Public defence
2009-11-20, Hörsalen, Klinisk Mikrobiologi, Akademiska Sjukhuset, Dag Hammarskjöldsväg 17, Uppsala, 09:15 (English)
Available from: 2009-10-30 Created: 2009-10-05 Last updated: 2009-10-30Bibliographically approved

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