Inhibition of splicing by serine-arginine rich protein 55 (SRp55) causes the appearance of partially spliced HIV-1 mRNAs in the cytoplasm
2011 (English)In: Virus Research, ISSN 0168-1702, E-ISSN 1872-7492, Vol. 157, no 1, 82-91 p.Article in journal (Refereed) Published
We have previously shown that SRp55 inhibits splicing from HIV-1 exon 3, thereby generating partially spliced mRNAs encoding HIV-1 vpr. Here we show that SRp55 also inhibits splicing from HIV-1 exon 5 to generate HIV-1 vpu/env mRNA, albeit with lower efficiency. We also show that inhibition of HIV-1 splicing by SRp55 causes the appearance of partially spliced vpu, env and vpr mRNAs in the cytoplasm. SRp55 could also induce production of extracellular p24gag from a rev-defective HIV-1 provirus. These results indicate that SRp55 aids in the generation of partially spliced and unspliced HIV-1 mRNAs.
Place, publisher, year, edition, pages
2011. Vol. 157, no 1, 82-91 p.
HIV-1, vpr, SRp55, Splicing
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-153549DOI: 10.1016/j.virusres.2011.02.010ISI: 000289864600011PubMedID: 21345357OAI: oai:DiVA.org:uu-153549DiVA: diva2:417256