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Comparison between Chicken and Rabbit Antibody Based Particle Enhanced Cystatin C Reagents for Immunoturbidimetry
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences. (Biokemisk struktur och funktion)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences. (Biokemisk struktur och funktion)
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Surface Biotechnology.
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2008 (English)In: Journal of immunoassay & immunochemistry, ISSN 1532-1819, E-ISSN 1532-4230, Vol. 29, no 1, 1-9 p.Article in journal (Refereed) Published
Abstract [en]

We have compared three commercial particle enhanced cystatin C reagents. One of the reagents utilizes chicken antibodies and the other two reagents are rabbit antibody based. We show that the chicken antibody based reagent yields a higher delta absorbance when reacting with the antigen. IgY coupled to latex particles show a strong scatter response even at high antigen concentrations in contrast to the steep decline in scatter previously reported for IgY antibodies in solution. The reagent also showed a low CV for duplicate samples. Laying hens thus seems as an interesting source of antibodies for particle-enhanced immunoassays.

Place, publisher, year, edition, pages
2008. Vol. 29, no 1, 1-9 p.
Keyword [en]
Chicken antibodies, IgY, Rabbit antibodies, Latex particles, Particle-enhanced immunoassays, Cystatin C
National Category
Physical Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-13980DOI: 10.1080/15321810701734644ISI: 000252219300001OAI: oai:DiVA.org:uu-13980DiVA: diva2:41750
Available from: 2008-01-28 Created: 2008-01-28 Last updated: 2017-12-11Bibliographically approved

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Caldwell, KarinFromell, Karin

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