Comparison between Chicken and Rabbit Antibody Based Particle Enhanced Cystatin C Reagents for Immunoturbidimetry
2008 (English)In: Journal of immunoassay & immunochemistry, ISSN 1532-1819, Vol. 29, no 1, 1-9 p.Article in journal (Refereed) Published
We have compared three commercial particle enhanced cystatin C reagents. One of the reagents utilizes chicken antibodies and the other two reagents are rabbit antibody based. We show that the chicken antibody based reagent yields a higher delta absorbance when reacting with the antigen. IgY coupled to latex particles show a strong scatter response even at high antigen concentrations in contrast to the steep decline in scatter previously reported for IgY antibodies in solution. The reagent also showed a low CV for duplicate samples. Laying hens thus seems as an interesting source of antibodies for particle-enhanced immunoassays.
Place, publisher, year, edition, pages
2008. Vol. 29, no 1, 1-9 p.
Chicken antibodies, IgY, Rabbit antibodies, Latex particles, Particle-enhanced immunoassays, Cystatin C
IdentifiersURN: urn:nbn:se:uu:diva-13980DOI: 10.1080/15321810701734644ISI: 000252219300001OAI: oai:DiVA.org:uu-13980DiVA: diva2:41750