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The Frog as a Model for Studies on Reproductive Toxicity of Progestagenic Environmental Pollutants
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology, Environmental Toxicology.
2011 (English)Licentiate thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Uppsala: Institutionen för organismbiologi , 2011.
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:uu:diva-154029OAI: oai:DiVA.org:uu-154029DiVA: diva2:418807
Available from: 2011-07-19 Created: 2011-05-24 Last updated: 2011-07-19Bibliographically approved
List of papers
1. Early life progestin exposure causes arrested oocyte development, oviductal agenesis and sterility in adult Xenopus tropicalis frogs
Open this publication in new window or tab >>Early life progestin exposure causes arrested oocyte development, oviductal agenesis and sterility in adult Xenopus tropicalis frogs
2011 (English)In: Aquatic Toxicology, ISSN 0166-445X, E-ISSN 1879-1514, Vol. 103, no 1-2, 18-24 p.Article in journal (Refereed) Published
Abstract [en]

Levonorgestrel (LNG) is a commonly used pharmaceutical progestin found in the environment. Information on the long-term toxicity of progestins following early life exposure is scant. We investigated the effects of developmental LNG exposure on sex differentiation, reproductive organ development and fertility in the model frog Xenopus tropicalis. Tadpoles were exposed to 0, 0.06 or 0.5 nM LNG via the water from hatching until metamorphosis. At metamorphosis effects on gonadal differentiation were evaluated using a subsample of frogs. Remaining animals were held unexposed for nine months, at which time reproductive organ structure, function and fertility were determined. LNG exposure severely impaired oviduct and ovary development and fertility. All adult females in the 0.5 nM group (n = 10) completely lacked oviducts. They also displayed a significantly larger fraction of immature oocytes, arrested in meiotic prophase, than control females. Upon mating with unexposed males, only one of 11 LNG-exposed females laid eggs, whereas all control females did. No effects on testicular development, sperm count or male fertility were observed. At metamorphosis, no effects on sex ratio or gonadal histology were evident. The effects on ovarian and oviductal development were detected at adult age but not at metamorphosis, emphasising the importance of investigating the long-term consequences of developmental exposure. This is the first developmental reproductive toxicity study of a progestin in an aquatic vertebrate. Considering that several progestins are present in contaminated surface waters, further investigation into the sensitivity of frogs to progestins is warranted to understand the risk such compounds may pose to wild frog populations.

Keyword
Endocrine disruption, Developmental reproductive toxicity, Levonorgestrel, Oogenesis, Oviduct, Sex differentiation
National Category
Biological Sciences
Research subject
Ecotoxicology
Identifiers
urn:nbn:se:uu:diva-153282 (URN)10.1016/j.aquatox.2011.02.003 (DOI)000290502000003 ()21392492 (PubMedID)
Available from: 2011-05-10 Created: 2011-05-10 Last updated: 2017-12-11Bibliographically approved
2. Inhibited oogenesis in Xenopus tropicalis frogs after exposure to levonorgestrel
Open this publication in new window or tab >>Inhibited oogenesis in Xenopus tropicalis frogs after exposure to levonorgestrel
(English)Manuscript (preprint) (Other academic)
Identifiers
urn:nbn:se:uu:diva-153327 (URN)
Available from: 2011-05-10 Created: 2011-05-10 Last updated: 2011-07-19

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