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One-step purification of epigallocatechin gallate from crude green tea extracts by mixed-mode adsorption chromatography on highly cross-linked agarose media
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Surface Biotechnology.
2007 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1169, no 1-2, 235-238 p.Article in journal (Refereed) Published
Abstract [en]

(−)-Epigallocatechin gallate (EGCG) was purified in one step from a green tea polyphenol (GTP) crude extract by adsorption chromatography on a Superose 12 HR 10/30 column. The mobile phase used was a mixture of acetonitrile and water with an optimum mobile phase compositions regarding purity, recovery and separation time of 78/22 (v/v). Maximum practical sample loading was 100 mg GTP per run (corresponding to 4.2 mg/ml Superose). An EGCG purity of 99% with recoveries in the range 60–65% was achieved in one step directly from the crude GTP extract. Full column regeneration was obtained using solvents in the following order: 0.5 M NaOH, distilled water and 30% acetic acid.

Place, publisher, year, edition, pages
2007. Vol. 1169, no 1-2, 235-238 p.
Keyword [en]
Green tea polyphenols, (−)-Epigallocatechin gallate, EGCG, Agarose gel media, Mixed-mode adsorption chromatography
National Category
Chemical Sciences
URN: urn:nbn:se:uu:diva-14215DOI: 10.1016/j.chroma.2007.08.085ISI: 000250316400024PubMedID: 17897657OAI: oai:DiVA.org:uu-14215DiVA: diva2:41985
Available from: 2008-01-29 Created: 2008-01-29 Last updated: 2011-01-20Bibliographically approved

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