Coupling oligo-β-cyclodextrin on polyacrylate beads media for separation of puerarin
2007 (English)In: Process Biochemistry, ISSN 1359-5113, E-ISSN 1873-3298, Vol. 42, no 7, 1075-1083 p.Article in journal (Refereed) Published
The isoflavonoid puerarin, a well-known bioactive constituent in traditional Chinese medicine, can be separated from other isoflavonoids in the extracts of Radix puerariae (root of the plant Pueraria lobata) on oligo-β-cyclodextrin ligand coupled to D152 beads. The parameters of coupling time, temperature, solvent system and molar ratio of reactants were tested to optimize the preparation conditions. Acetic acid (7%) was used as the optimum mobile phase. The fine particle sizes of the coupled microspheres packing displayed high resolving power and efficiency. Compared with other two polyacrylate carrier media, PGMA-CDP and PHEMA-CDP, various retention mechanisms such as β-CD inclusion complexation, hydrogen bond interaction, and π–π interaction might exist in the chromatography process. This study provides a novel alternative matrix for the separation of puerarin.
Place, publisher, year, edition, pages
2007. Vol. 42, no 7, 1075-1083 p.
β-Cyclodextrin, Chromatography, Polyacrylate beads media, Coupling, Separation, Puerarin
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:uu:diva-14217DOI: 10.1016/j.procbio.2007.04.008ISI: 000248470800006OAI: oai:DiVA.org:uu-14217DiVA: diva2:41987