uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Coupling oligo-β-cyclodextrin on polyacrylate beads media for separation of puerarin
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Surface Biotechnology.
2007 (English)In: Process Biochemistry, ISSN 1359-5113, E-ISSN 1873-3298, Vol. 42, no 7, 1075-1083 p.Article in journal (Refereed) Published
Abstract [en]

The isoflavonoid puerarin, a well-known bioactive constituent in traditional Chinese medicine, can be separated from other isoflavonoids in the extracts of Radix puerariae (root of the plant Pueraria lobata) on oligo-β-cyclodextrin ligand coupled to D152 beads. The parameters of coupling time, temperature, solvent system and molar ratio of reactants were tested to optimize the preparation conditions. Acetic acid (7%) was used as the optimum mobile phase. The fine particle sizes of the coupled microspheres packing displayed high resolving power and efficiency. Compared with other two polyacrylate carrier media, PGMA-CDP and PHEMA-CDP, various retention mechanisms such as β-CD inclusion complexation, hydrogen bond interaction, and π–π interaction might exist in the chromatography process. This study provides a novel alternative matrix for the separation of puerarin.

Place, publisher, year, edition, pages
2007. Vol. 42, no 7, 1075-1083 p.
Keyword [en]
β-Cyclodextrin, Chromatography, Polyacrylate beads media, Coupling, Separation, Puerarin
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:uu:diva-14217DOI: 10.1016/j.procbio.2007.04.008ISI: 000248470800006OAI: oai:DiVA.org:uu-14217DiVA: diva2:41987
Available from: 2008-01-29 Created: 2008-01-29 Last updated: 2011-01-26Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Janson, Jan-Christer
By organisation
Surface Biotechnology
In the same journal
Process Biochemistry
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 197 hits
ReferencesLink to record
Permanent link

Direct link