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The three-dimensional structure of ryegrass mottle virus at 2.9 Å resolution
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Structural Molecular Biology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Structural Molecular Biology.
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2007 (English)In: Virology, ISSN 0042-6822, E-ISSN 1096-0341, Vol. 369, no 2, 364-374 p.Article in journal (Refereed) Published
Abstract [en]

The crystal structure of the sobemovirus Ryegrass mottle virus (RGMoV) has been determined at 2.9 Å resolution. The coat protein has a canonical jellyroll β-sandwich fold. In comparison to other sobemoviruses the RGMoV coat protein is missing several residues in two of the loop regions. The first loop contributes to contacts between subunits around the quasi-threefold symmetry axis. The altered contact interface results in tilting of the subunits towards the quasi-threefold axis. The assembly of the T = 3 capsid of sobemoviruses is controlled by the N-termini of C subunits forming a so-called β-annulus. The other loop that is smaller in the RGMoV structure contains a helix that participates in stabilization of the β-annulus in other sobemoviruses. The loss of interaction between the RGMoV loop and the β-annulus has been compensated for by additional interactions between the N-terminal arms. As a consequence of these differences, the diameter of the RGMoV particle is 8 Å smaller than that of the other sobemoviruses.

The interactions of coat proteins in sobemovirus capsids involve calcium ions. Depletion of calcium ions results in particle swelling, which is considered a first step in disassembly. We could not identify any density for metal ions in the proximity of the conserved residues normally involved in calcium binding, but the RGMoV structure does not show any signs of swelling. A likely reason is the low pH (3.0) of the crystallization buffer in which the groups interacting with the calcium ions are not charged.

Place, publisher, year, edition, pages
2007. Vol. 369, no 2, 364-374 p.
Keyword [en]
Ryegrass mottle virus, Sobemovirus, Virus structure, Virus assembly
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:uu:diva-14218DOI: 10.1016/j.virol.2007.07.028ISI: 000251263200013PubMedID: 17881031OAI: oai:DiVA.org:uu-14218DiVA: diva2:41988
Available from: 2008-01-29 Created: 2008-01-29 Last updated: 2017-12-11Bibliographically approved

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Plevka, PavelLiljas, Lars

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