Assessment of 4-nitro-1,8-naphthalic anhydride reductase activity in homogenates of bakers' yeast by reversed-phase high-performance liquid chromatography
2007 (English)In: Journal of chromatography. B, ISSN 1570-0232, E-ISSN 1873-376X, Vol. 847, no 2, 82-87 p.Article in journal (Refereed) Published
A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method was developed for the simultaneous determination of yield and conversion ratio of 4-nitro-1,8-naphthalic anhydride to 4-amino-1,8-naphthalic anhydride following incubation with a crude bakers’ yeast homogenate. The analytes were separated on a C18 column in gradient mode. The detection limit of 4-amino-1,8-naphthalic anhydride is 10 ng/μl when using a 10 μl sample injection volume. The nitroreductase activity in the homogenate system can be assessed during the bioconversion process. The method can be used for the simultaneous detection of 4-hydroxylamino-1,8-naphthalic anhydride, an intermediate with limited stability.
Place, publisher, year, edition, pages
2007. Vol. 847, no 2, 82-87 p.
Nitroaryl, Nitroarene, Arylhydroxylamine, Aminoaryl, Bakers’ yeast, Nitroarylreductase, HPLC, TLC
IdentifiersURN: urn:nbn:se:uu:diva-14226DOI: 10.1016/j.jchromb.2006.09.040ISI: 000245387600003PubMedID: 17070119OAI: oai:DiVA.org:uu-14226DiVA: diva2:41996