uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Evolution of the cutinase gene family: Evidence for lateral gene transfer of a candidate Phytophthora virulence factor
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Microbiology. (Andersson)
2008 (English)In: Gene, ISSN 0378-1119, E-ISSN 1879-0038, Vol. 408, no 1-2, 1-8 p.Article in journal (Refereed) Published
Abstract [en]

Lateral gene transfer (LGT) can facilitate the acquisition of new functions in recipient lineages, which may enable them to colonize new environments. Several recent publications have shown that gene transfer between prokaryotes and eukaryotes occurs with appreciable frequency. Here we present a study of interdomain gene transfer of cutinases -- well documented virulence factors in fungi -- between eukaryotic plant pathogens Phytophthora species and prokaryotic bacterial lineages. Two putative cutinase genes were cloned from Phytophthora brassicae and Northern blotting experiments showed that these genes are expressed early during the infection of the host Arabidopsis thaliana and induced during cyst germination of the pathogen. Analysis of the gene organisation of this gene family in Phytophthora ramorum and P. sojae showed three and ten copies in tight succession within a region of 5 and 25 kb, respectively, probably indicating a recent expansion in Phytophthora lineages by gene duplications. Bioinformatic analyses identified orthologues only in three genera of Actinobacteria, and in two distantly related eukaryotic groups: oomycetes and fungi. Together with phylogenetic analyses this limited distribution of the gene in the tree of life strongly support a scenario where cutinase genes originated after the origin of land plants in a microbial lineage living in proximity of plants and subsequently were transferred between distantly related plant-degrading microbes. More precisely, a cutinase gene was likely acquired by an ancestor of P. brassicae, P. sojae, P. infestans and P. ramorum, possibly from an actinobacterial source, suggesting that gene transfer might be an important mechanism in the evolution of their virulence. These findings could indeed provide an interesting model system to study acquisition of virulence factors in these important plant pathogens.

Place, publisher, year, edition, pages
2008. Vol. 408, no 1-2, 1-8 p.
Keyword [en]
Phytophthora spp., Cutinase, Arabidopsis, Lateral gene transfer
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:uu:diva-14229DOI: 10.1016/j.gene.2007.10.019ISI: 000253033600001PubMedID: 18024004OAI: oai:DiVA.org:uu-14229DiVA: diva2:41999
Available from: 2008-01-29 Created: 2008-01-29 Last updated: 2011-02-16Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Andersson, Jan O
By organisation
In the same journal
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 565 hits
ReferencesLink to record
Permanent link

Direct link