One-step purification of resveratrol and polydatin from Polygonum cuspidatum (Sieb. & Zucc.) by isocratic hydrogen bond adsorption chromatography on cross-linked 12% agarose
2006 (English)In: Chromatographia, ISSN 0009-5893, E-ISSN 1612-1112, Vol. 64, no 11-12, 701-704 p.Article in journal (Refereed) Published
Resveratrol and polydatin (piceid), the major active components of the traditional Chinese medicinal herb Polygonum cuspidatum (Sieb. & Zucc.), have been separated and purified from crude root extracts in one step by isocratic hydrogen bond adsorption chromatography on cross-linked 12% agarose (Superose™ 12 HR 10/30). Separation was achieved by step-wise elution with mobile phases composed of mixtures of ethanol and acetic acid: 0–75 mL, 10% ethanol, 10% acetic acid; 75–150 mL, 20% ethanol, 20% acetic acid; 150–250 mL, 30% ethanol, 30% acetic acid. At a sample load of 40 mg crude extract dissolved in 0.5 mL mobile phase (corresponding to a load of 1.7 mg mL−1 gel) a resveratrol purity of about 96% with a recovery of 61% was obtained by proper peak cutting.
Place, publisher, year, edition, pages
2006. Vol. 64, no 11-12, 701-704 p.
column liquid chromatography, hydrogen bonded adsorption chromatography, resveratrol and polydatin
IdentifiersURN: urn:nbn:se:uu:diva-14232DOI: 10.1365/s10337-006-0081-xISI: 000243066200013OAI: oai:DiVA.org:uu-14232DiVA: diva2:42002