One-step simultaneous purification of three water-soluble constituents in crude extracts from Danshen by adsorption chromatography on oligo-β-cyclodextrin substituted agarose gel media
2007 (English)In: Process Biochemistry, ISSN 1359-5113, E-ISSN 1873-3298, Vol. 42, no 3, 480-485 p.Article in journal (Refereed) Published
A new adsorption chromatographic method for the simultaneous purification of Danshensu, Protocatechuic aldehyde and Salvianolic acid B (Lithospermic acid B) from a crude extract from radix of Danshen (Salviae miltiorrhiza) has been developed using oligo-β-cyclodextrin immobilized to Sepharose HP agarose gel media. Sample loads of up to 2.3 mg crude extracts/ml gel gave satisfactory results. The target components were eluted using step-wise isocratic elution with increasing concentrations of acetic acid. The separated fractions were identified by LC–MS. The recoveries of Danshensu, Protocatechuic aldehyde and Salvianolic acid B were 88.3%, 90.2% and 73.6% with purities of 99%, 99%, and 98%, respectively. Isocratic elution in 6% acetic acid gave baseline separation of Danshensu and Protocatechuic aldehyde, whereas 40% acetic acid was required for purification of Salvianolic acid B. Full separation efficiency could be restored by cleaning the column with 0.5 M NaOH followed by distilled water and 30% acetic acid.
Place, publisher, year, edition, pages
2007. Vol. 42, no 3, 480-485 p.
β-Cyclodextrin, Salviae miltiorrhizae, Danshensu, Protocatechuic aldehyde, Salvianolic acid B, Lithospermic acid B, Agarose gel media
IdentifiersURN: urn:nbn:se:uu:diva-14238DOI: 10.1016/j.procbio.2006.09.015ISI: 000245021300028OAI: oai:DiVA.org:uu-14238DiVA: diva2:42008