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PTAA and B10: new approaches to amyloid detection in tissue-evaluation of amyloid detection in tissue with a conjugated polyelectrolyte and a fibril-specific antibody fragment
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
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2011 (English)In: Amyloid: Journal of Protein Folding Disorders, ISSN 1350-6129, Vol. 18, no 2, 47-52 p.Article in journal (Refereed) Published
Abstract [en]

Methods: aEuro integral We compared the amyloid detection of PTAA and B10 to Congo red in 106 amyloid-containing tissue biopsies of diverse anatomical and precursor origin by evaluating the accordance in four grades (grade 0: no staining, grade 1: staining of < 33%% of the amyloid deposits, grade 2: 33--66%% and grade 3: aEuroS > 66%%). Results: aEuro integral PTAA showed grade 2--3 staining in 57 (54%%) cases, while B10 presented this accordance in only 25 (24%%) tissue biopsies. Grade 1 staining was found in 11 (10%%) samples with PTAA and in 62 (58%%) cases with B10. No staining at all (grade 0) occurred in 38 (36%%) biopsies when using PTAA and in 19 (18%%) cases when using B10. Conclusion: aEuro integral Although conformation-sensitive detection seemed promising, PTAA and B10 stain only a fraction of the examined amyloid samples when using routine surgical pathology settings. This study emphasises the necessity of having optimised pre-analytical protocols for recovery, storage and handling of samples if these novel amyloid ligands are to be used in routine diagnosis of amyloid.

Place, publisher, year, edition, pages
2011. Vol. 18, no 2, 47-52 p.
Keyword [en]
amyloid, PTAA, camelid antibody
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-154351DOI: 10.3109/13506129.2011.560623ISI: 000290486500002PubMedID: 21401323OAI: oai:DiVA.org:uu-154351DiVA: diva2:420093
Available from: 2011-05-31 Created: 2011-05-31 Last updated: 2011-05-31Bibliographically approved

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