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The Separation of Polyphenols by Isocratic Hydrogen Bond Adsorption Chromatography on a Cross-Linked 12% Agarose Gel
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Surface Biotechnology.
2006 (English)In: Chromatographia, ISSN 0009-5893, E-ISSN 1612-1112, Vol. 64, no 5-6, 247-253 p.Article in journal (Refereed) Published
Abstract [en]

The highly cross-linked 12% agarose gel, Superoseā„¢ 12 HR 10/30 is shown to possess hydrogen bond acceptor properties suitable for the separation of polyphenolic solutes such as phenolic acids, flavonols and flavonoids. The separation is achieved isocratically in the presence of solvent mixtures of acetic acid and ethanol. The extent of hydrogen bond adsorption is reviewed based on data obtained from the elution behaviour of a variety of simple polyphenolic solutes including dihydroxybenzoic acids.

Place, publisher, year, edition, pages
2006. Vol. 64, no 5-6, 247-253 p.
Keyword [en]
column liquid chromatography, hydrogen bond adsorption chromatography, polyphenols, agarose gel
National Category
Physical Chemistry
URN: urn:nbn:se:uu:diva-14245DOI: 10.1365/s10337-006-0039-zISI: 000241222200003OAI: oai:DiVA.org:uu-14245DiVA: diva2:42015
Available from: 2008-01-29 Created: 2008-01-29 Last updated: 2011-05-31Bibliographically approved

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Janson, Jan-Christer
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Surface Biotechnology
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