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Chromatographic retention of epigallocatechin gallate on oligo-beta-cycloclextrin coupled sepharose media investigated using NMR
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Surface Biotechnology.
2006 (English)In: Chromatographia, ISSN 0009-5893, E-ISSN 1612-1112, Vol. 64, no 1-2, 7-11 p.Article in journal (Refereed) Published
Abstract [en]

The retention of epigallocatechin gallate (EGCG) on oligo-β-cyclodextrin (oligo-β-CD) bonded agarose chromatographic media was investigated. NMR spectroscopy in solution showed that the EGCG immerses into the β-CD cavity. The association constant calculated by NMR titration was used to estimate a retention factor which accurately reflected chromatographic behaviour. This correlation suggests that oligo-β-CD forms inclusion complexes with EGCG via the same mechanism as monomeric β-CD.

Place, publisher, year, edition, pages
2006. Vol. 64, no 1-2, 7-11 p.
Keyword [en]
column liquid chromatography, nuclear magnetic resonance, β-Cylcodextrin-bonded stationary phases, epigallocatechin gallate, agarose
National Category
Physical Chemistry
URN: urn:nbn:se:uu:diva-14248DOI: 10.1365/s10337-006-0819-5ISI: 000239677700002OAI: oai:DiVA.org:uu-14248DiVA: diva2:42018
Available from: 2008-01-29 Created: 2008-01-29 Last updated: 2011-06-29Bibliographically approved

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Janson, Jan-Christer
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Surface Biotechnology
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