uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Design, Synthesis, and Characterization of a Highly Effective Hog1 Inhibitor: A Powerful Tool for Analyzing MAP Kinase Signaling in Yeast
University of Gothenburg. (Morten Grøtli)
Show others and affiliations
2011 (English)In: PLoS ONE, Vol. 6, no 5, e20012- p.Article in journal (Refereed) Published
Abstract [en]

The Saccharomyces cerevisiaeHigh-Osmolarity Glycerol (HOG) pathway is a conserved mitogen-activated protein kinase (MAPK) signal transduction system that often serves as a model to analyze systems level properties of MAPK signaling. Hog1, the MAPK of the HOG-pathway, can be activated by various environmental cues and it controls transcription, translation, transport, and cell cycle adaptations in response to stress conditions. A powerful means to study signaling in living cells is to use kinase inhibitors; however, no inhibitor targeting wild-type Hog1 exists to date. Herein, we describe the design, synthesis, and biological application of small molecule inhibitors that are cell-permeable, fast-acting, and highly efficient against wild-type Hog1. These compounds are potent inhibitors of Hog1 kinase activity both in vitroand in vivo. Next, we use these novel inhibitors to pinpoint the time of Hog1 action during recovery from G1 checkpoint arrest, providing further evidence for a specific role of Hog1 in regulating cell cycle resumption during arsenite stress. Hence, we describe a novel tool for chemical genetic analysis of MAPK signaling and provide novel insights into Hog1 action.

Place, publisher, year, edition, pages
2011. Vol. 6, no 5, e20012- p.
National Category
Organic Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-154405DOI: 10.1371/journal.pone.0020012OAI: oai:DiVA.org:uu-154405DiVA: diva2:420308
Note
internal-pdf://journal-2.pone.0020012-0326142720/journal-2.pone.0020012.pdfAvailable from: 2011-06-01 Created: 2011-06-01 Last updated: 2011-11-29

Open Access in DiVA

No full text

Other links

Publisher's full texthttp://www.plosone.org/article/info:doi/10.1371/journal.pone.0020012

Authority records BETA

Dinér, Peter

Search in DiVA

By author/editor
Dinér, Peter
Organic Chemistry

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 384 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf