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Characterization of photophysical and base-mimicking properties of a novel fluorescent adenine analogue in DNA.
University of Gothenburg. (Morten Grøtli)
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2011 (English)In: Nucleic Acids Research, ISSN 0305-1048, E-ISSN 1362-4962, Vol. 39, no 10, 4513-24 p.Article in journal (Refereed) Published
Abstract [en]

To increase the diversity of fluorescent base analogues with improved properties, we here present the straightforward click-chemistry-based synthesis of a novel fluorescent adenine-analogue triazole adenine (A(T)) and its photophysical characterization inside DNA. A(T) shows promising properties compared to the widely used adenine analogue 2-aminopurine. Quantum yields reach >20% and >5% in single- and double-stranded DNA, respectively, and show dependence on neighbouring bases. Moreover, A(T) shows only a minor destabilization of DNA duplexes, comparable to 2-aminopurine, and circular dichroism investigations suggest that A(T) only causes minimal structural perturbations to normal B-DNA. Furthermore, we find that A(T) shows favourable base-pairing properties with thymine and more surprisingly also with normal adenine. In conclusion, A(T) shows strong potential as a new fluorescent adenine analogue for monitoring changes within its microenvironment in DNA.

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2011. Vol. 39, no 10, 4513-24 p.
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URN: urn:nbn:se:uu:diva-154406DOI: 10.1093/nar/gkr010PubMedID: 21278417OAI: oai:DiVA.org:uu-154406DiVA: diva2:420309
Available from: 2011-06-01 Created: 2011-06-01 Last updated: 2017-12-11

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