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Molecular characterization of recombinant Hepatitis B surface antigen from Chinese hamster ovary and Hansenula polymorpha cells by high-performance size exclusion chromatography and multi-angle laser light scattering
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Surface Biotechnology. Ytbioteknik.
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2006 (English)In: Journal of chromatography. B, ISSN 1570-0232, E-ISSN 1873-376X, Vol. 838, no 2, 71-77 p.Article in journal (Refereed) Published
Abstract [en]

The molecular weight and size of recombinant Hepatitis B surface antigen (HBsAg) derived from Chinese hamster ovary (CHO) and the Hansenula polymorph have been characterized by high-performance size exclusion chromatography with multi-angle laser light scattering (HPSEC-MALLS). The average molecular weight of CHO-derived HBsAg particle (CHO-rHBsAg) (4921 kDa) was higher than that of H. polymorpha yeast strain (Hans-rHBsAg) (3010 kDa). The size of CHO-rHBsAg (22.1 nm) is nearly the same as that of native HBsAg compared to 18.1 nm for Hans-rHBsAg. The average monomer numbers were found to be 155 for CHO-rHBsAg and 86 for Hans-rHBsAg, respectively. The data obtained support the assumption that the higher immunogenicity of CHO-derived HBsAg is related to its more favorable macromolecular assembly structure.

Place, publisher, year, edition, pages
2006. Vol. 838, no 2, 71-77 p.
Keyword [en]
hepatitis B surface antigen (HBsAg), molecular weight, molecular size, distribution, HPSEC-MALLS
National Category
Physical Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-14265DOI: 10.1016/j.jchromb.2006.03.064ISI: 000238963400001PubMedID: 16757217OAI: oai:DiVA.org:uu-14265DiVA: diva2:42035
Available from: 2008-01-29 Created: 2008-01-29 Last updated: 2017-12-11Bibliographically approved

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Janson, Jan-Christer

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