uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
One-step separation and purification of hydrolysable tannins from Geranium wilfordii Maxim by adsorption chromatography on cross-linked 12% agarose gel
Show others and affiliations
2011 (English)In: Journal of Separation Science, ISSN 1615-9306, E-ISSN 1615-9314, Vol. 34, no 9, 995-998 p.Article in journal (Refereed) Published
Abstract [en]

The hydrolysable tannins corilagin and geraniin, the major active components of the traditional Chinese medicine Geranium wilfordii Maxim, have been separated and purified from crude extracts in one step by adsorption chromatography on cross-linked 12% agarose gel (Superose 12 10/300 GL). The separation was achieved by gradient elution using mobile phase A composed of 5% ethanol and 5% acetic acid and mobile phase B composed of 30% ethanol and 30% acetic acid. The gradients were composed as follows: 0-240 mL, 0-25% B; 240-480 mL, 25-40% B; after 480 mL, 100% B. The purities of the collected corilagin and geraniin were 92.4 and 87.2%, and the corresponding yields were 88.0 and 76.8%, respectively.

Place, publisher, year, edition, pages
2011. Vol. 34, no 9, 995-998 p.
Keyword [en]
Adsorption chromatography, Cross-linked agarose gel, Hydrolysable tannin
National Category
Chemical Sciences
URN: urn:nbn:se:uu:diva-154541DOI: 10.1002/jssc.201100006ISI: 000290634600004PubMedID: 21442751OAI: oai:DiVA.org:uu-154541DiVA: diva2:420989
Available from: 2011-06-07 Created: 2011-06-07 Last updated: 2011-06-07Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed
By organisation
Analytical Chemistry
In the same journal
Journal of Separation Science
Chemical Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 404 hits
ReferencesLink to record
Permanent link

Direct link