Neurotransmitter phenotype-specific expression changes in developing sympathetic neurons
2007 (English)In: Molecular and Cellular Neuroscience, ISSN 1044-7431, E-ISSN 1095-9327, Vol. 35, no 3, 397-408 p.Article in journal (Refereed) Published
During late developmental phases individual sympathetic neurons undergo a switch from noradrenergic to cholinergic neurotransmission. This phenomenon of plasticity depends on target-derived signals in vivo and is triggered by neurotrophic factors in neuronal cultures. To analyze genome-wide expression differences between the two transmitter phenotypes we employed DNA microarrays. RNA expression profiles were obtained from chick paravertebral sympathetic ganglia, treated with neurotrophin 3, glial cell line-derived neurotrophic factor or ciliary neurotrophic factor, all of which stimulate cholinergic differentiation. Results were compared with the effect of nerve growth factor, which functions as a pro-noradrenergic stimulus. The gene set common to all three comparisons defined the noradrenergic and cholinergic synexpression groups. Several functional categories, such as signal transduction, G-protein-coupled signaling, cation transport, neurogenesis and synaptic transmission, were enriched in these groups. Experiments based on the prediction that some of the identified genes play a role in the neurotransmitter switch identified bone morphogenetic protein signaling as an inhibitor of cholinergic differentiation.
Place, publisher, year, edition, pages
2007. Vol. 35, no 3, 397-408 p.
Acetylcholine, Noradrenalin, Sympathetic, Neurotrophic, Microarray
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-14354DOI: 10.1016/j.mcn.2007.03.014ISI: 000248057600001PubMedID: 17513123OAI: oai:DiVA.org:uu-14354DiVA: diva2:42124