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Molecular diagnostics using magnetic nanobeads
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Solid State Physics.
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2010 (English)In: / [ed] Goll G., Lohneysen H.V., Loidl A., Pruschke T., Richter M., Schultz L., Surgers C., Wosnitza J, 2010, Vol. 200, p. 122011-Conference paper, Published paper (Refereed)
Abstract [en]

In this paper, we investigate the volume-amplified magnetic nanobead detection assay with respect to bead size, bead concentration and bead oligonucleotide surface coverage in order to improve the understanding of the underlying microscopic mechanisms. It has been shown that: (i) the immobilization efficiency of the beads depends on the surface coverage of oligonucleotides, (ii) by using lower amounts of probe-tagged beads, detection sensitivity can be improved and (iii) using small enough beads enables both turn-off and turn-on detection. Finally, biplex detection was demonstrated.

Place, publisher, year, edition, pages
2010. Vol. 200, p. 122011-
Series
Journal of Physics: Conference Series, ISSN 1742-6588 ; 200
National Category
Natural Sciences Engineering and Technology
Identifiers
URN: urn:nbn:se:uu:diva-155696DOI: 10.1088/1742-6596/200/12/122011ISI: 000291321303110OAI: oai:DiVA.org:uu-155696DiVA, id: diva2:427669
Conference
International Conference on Magnetism (ICM 2009), Karlsruhe, Germany, JUL 26-31, 2009
Available from: 2011-06-28 Created: 2011-06-28 Last updated: 2016-11-30Bibliographically approved

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de la Torre, Teresa Zardan GomezStrömberg, MattiasGunnarsson, KlasSvedlindh, PeterStrømme, Maria

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de la Torre, Teresa Zardan GomezStrömberg, MattiasGunnarsson, KlasSvedlindh, PeterStrømme, Maria
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