Molecular diagnostics using magnetic nanobeads
2010 (English)In: / [ed] Goll G., Lohneysen H.V., Loidl A., Pruschke T., Richter M., Schultz L., Surgers C., Wosnitza J, 2010, Vol. 200, 122011- p.Conference paper (Refereed)
In this paper, we investigate the volume-amplified magnetic nanobead detection assay with respect to bead size, bead concentration and bead oligonucleotide surface coverage in order to improve the understanding of the underlying microscopic mechanisms. It has been shown that: (i) the immobilization efficiency of the beads depends on the surface coverage of oligonucleotides, (ii) by using lower amounts of probe-tagged beads, detection sensitivity can be improved and (iii) using small enough beads enables both turn-off and turn-on detection. Finally, biplex detection was demonstrated.
Place, publisher, year, edition, pages
2010. Vol. 200, 122011- p.
, Journal of Physics: Conference Series, ISSN 1742-6588 ; 200
Natural Sciences Engineering and Technology
IdentifiersURN: urn:nbn:se:uu:diva-155696DOI: 10.1088/1742-6596/200/12/122011ISI: 000291321303110OAI: oai:DiVA.org:uu-155696DiVA: diva2:427669
International Conference on Magnetism (ICM 2009), Karlsruhe, Germany, JUL 26-31, 2009