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Functional characterisation in vitro of an Affibody® molecule aimed for vascular targeted tumour therapy
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre.
2011 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

In a certain type of cancer immunotherapy, IL-2 is administered in high doses to the patient in order to trigger an immune response against tumour cells. The high dose administered leads to high general toxicity. By targeting IL-2 to the sites of the tumours, the dose can be decreased and toxicity reduced. Here, a PDGFR-! specific fusion protein (Z03358-ABD-IL2) aimed for delivery of IL-2 to tumour vasculature, has been characterised in vitro for IL-2-activity and target binding. Also, two PDGFR-! specific Affibody molecules (Z02465 and Z02483) have been characterised in vitro for target binding and biological blocking activity. Results showed that Z03358-ABD-IL2 had full IL-2 activity, but decreased target binding. In contrast, full target binding and blocking capacity was confirmed with Z02465 and Z02483. From this work it can be concluded that Z03358-ABD-IL2 has activity in all parts, but re-cloning with Z02465 or Z02483 should be considered for full target binding capacity.

Place, publisher, year, edition, pages
2011. , 36 p.
Keyword [en]
Cancer, tumour vasculature, tumour targeting, IL-2, Affibody molecule, pericyte, PDGFR-!
URN: urn:nbn:se:uu:diva-155895OAI: oai:DiVA.org:uu-155895DiVA: diva2:429146
Educational program
Molecular Biotechnology Engineering Programme
Physics, Chemistry, Mathematics
Available from: 2011-07-05 Created: 2011-07-03 Last updated: 2011-07-05Bibliographically approved

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