Bacteria-Induced Dscam Isoforms of the Crustacean, Pacifastacus leniusculus
2011 (English)In: PLoS Pathogens, ISSN 1553-7366, E-ISSN 1553-7374, Vol. 7, no 6, e1002062Article in journal (Refereed) Published
The Down syndrome cell adhesion molecule, also known as Dscam, is a member of the immunoglobulin super family. Dscam plays an essential function in neuronal wiring and appears to be involved in innate immune reactions in insects. The deduced amino acid sequence of Dscam in the crustacean Pacifastacus leniusculus (PlDscam), encodes 9(Ig)-4(FNIII)-(Ig)-2(FNIII)-TM and it has variable regions in the N-terminal half of Ig2 and Ig3 and the complete Ig7 and in the transmembrane domain. The cytoplasmic tail can generate multiple isoforms. PlDscam can generate more than 22,000 different unique isoforms. Bacteria and LPS injection enhanced the expression of PlDscam, but no response in expression occurred after a white spot syndrome virus (WSSV) infection or injection with peptidoglycans. Furthermore, PlDscam silencing did not have any effect on the replication of the WSSV. Bacterial specific isoforms of PlDscam were shown to have a specific binding property to each tested bacteria, E. coli or S. aureus. The bacteria specific isoforms of PlDscam were shown to be associated with bacterial clearance and phagocytosis in crayfish.
Place, publisher, year, edition, pages
2011. Vol. 7, no 6, e1002062
IdentifiersURN: urn:nbn:se:uu:diva-156026DOI: 10.1371/journal.ppat.1002062ISI: 000292379600004PubMedID: 21695245OAI: oai:DiVA.org:uu-156026DiVA: diva2:429850
This article is in the process of being retracted.2011-07-062011-07-062016-04-29Bibliographically approved