A new mouse line based on the Gpr101 promoter drives expression of Cre in medium spiny neurons of the striatum.
(English)Manuscript (preprint) (Other academic)
A novel transgenic mouse that expresses the Cre recombinase in striatal medium spiny neurons was generated. To create the line, we have used the promoter of the X-linked gene Gpr101 and a Bacterial Artificial Chromosome recombineering strategy. When the Gpr101-Cre mouse was bred to the tdTomato Ai14  reporter line, we observed strong fluorescence in medium-size spiny neurons (MSNs) of the striatum. In addition, Gpr101-Cre was detected in hippocampal pyramidal neurons and sparse cerebellar purkinje cells. Interestingly, Gpr101-Cre expression in this mouse line differs from the endogenous Gpr101 gene expression, which is highest in amygdala and hypothalamus and not detected in striatum in adult mice, as shown by in situ hybridization. The tdTomato Ai14 reporter marks any cell lineages in which Gpr101-Cre has, at any time, been expressed. Our results show that the Gpr101-Cre gene construct had lost the original ability of the Gpr101 promotor to drive expression of the Gpr101 gene in the amygdala and the hypothalamus. Second, the Gpr101-Cre gene construct either acquired a novel capability to express in striatum, or more probably, Gpr101 is expressed transiently in striatum during development. In addition, a small subpopulation of astrocytes (GFAP positive cells) was labelled in several regions of the central nervous system, allowing for specific follow-up studies of these cells. We envision that the newly created Cre-line will contribute to numerous studies, particularly related to the development and differentiation of cellular networks in the brain.
IdentifiersURN: urn:nbn:se:uu:diva-156638OAI: oai:DiVA.org:uu-156638DiVA: diva2:432660