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Bone reservoir: Injectable hyaluronic acid hydrogel for minimal invasive bone augmentation
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Materials Chemistry, Polymer Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Materials Chemistry, Polymer Chemistry.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Orthopaedics.
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2011 (English)In: Journal of Controlled Release, ISSN 0168-3659, E-ISSN 1873-4995, Vol. 152, no 2, 232-240 p.Article in journal (Refereed) Published
Abstract [en]

A strategy has been designed to develop hyaluronic acid (HA) hydrogel for in vivo bone augmentation using minimal invasive technique. A mild synthetic procedure was developed to prepare aldehyde modified HA by incorporating an amino-glycerol side chain via amidation reaction and selective oxidation of the pendent group. This modification, upon mixing with hydrazide modified HA formed hydrazone-crosslinked hydrogel within 30 s that was stable at physiological pH. In vitro experiments showed no cytotoxicity of hydrogel with the controlled release of active bone morphogenic protein-2 (BMP-2). In vivo evaluation of this gel as a BMP-2 carrier was performed by injecting gels over the rat calvarium and showed bone formation in 8 weeks in correlation with the amount of BMP-2 loaded (0, 1 and 30 pig) within the gel. Furthermore, hydrogels with 30 Kg of BMP-2 induced less bone formation upon subcutaneous injection in comparison with subperiosteal implantation. Histological examination showed newly formed bone with a high expression of osteocalcin, osteopontin and with angiogenic bone marrow when higher BMP-2 concentration was employed. Our result suggests that novel HA hydrogels could be used as a BMP-2 carrier and can promote bone augmentation for potential orthopedic applications.

Place, publisher, year, edition, pages
2011. Vol. 152, no 2, 232-240 p.
Keyword [en]
BMP (bone morphogenetic protein), Bone tissue engineering, Hyaluronic acid, Hydrogel, In vivo, Controlled release
National Category
Surgery Chemical Sciences Polymer Chemistry
Research subject
Chemistry with specialization in Polymer Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-156682DOI: 10.1016/j.jconrel.2011.02.003ISI: 000292665200003OAI: oai:DiVA.org:uu-156682DiVA: diva2:433371
Available from: 2011-08-09 Created: 2011-08-07 Last updated: 2017-12-08

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Ossipov, Dmitri A.Hilborn, JönsVarghese, Oommen P.

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