Bone reservoir: Injectable hyaluronic acid hydrogel for minimal invasive bone augmentation
2011 (English)In: Journal of Controlled Release, ISSN 0168-3659, E-ISSN 1873-4995, Vol. 152, no 2, 232-240 p.Article in journal (Refereed) Published
A strategy has been designed to develop hyaluronic acid (HA) hydrogel for in vivo bone augmentation using minimal invasive technique. A mild synthetic procedure was developed to prepare aldehyde modified HA by incorporating an amino-glycerol side chain via amidation reaction and selective oxidation of the pendent group. This modification, upon mixing with hydrazide modified HA formed hydrazone-crosslinked hydrogel within 30 s that was stable at physiological pH. In vitro experiments showed no cytotoxicity of hydrogel with the controlled release of active bone morphogenic protein-2 (BMP-2). In vivo evaluation of this gel as a BMP-2 carrier was performed by injecting gels over the rat calvarium and showed bone formation in 8 weeks in correlation with the amount of BMP-2 loaded (0, 1 and 30 pig) within the gel. Furthermore, hydrogels with 30 Kg of BMP-2 induced less bone formation upon subcutaneous injection in comparison with subperiosteal implantation. Histological examination showed newly formed bone with a high expression of osteocalcin, osteopontin and with angiogenic bone marrow when higher BMP-2 concentration was employed. Our result suggests that novel HA hydrogels could be used as a BMP-2 carrier and can promote bone augmentation for potential orthopedic applications.
Place, publisher, year, edition, pages
2011. Vol. 152, no 2, 232-240 p.
BMP (bone morphogenetic protein), Bone tissue engineering, Hyaluronic acid, Hydrogel, In vivo, Controlled release
Surgery Chemical Sciences Polymer Chemistry
Research subject Chemistry with specialization in Polymer Chemistry
IdentifiersURN: urn:nbn:se:uu:diva-156682DOI: 10.1016/j.jconrel.2011.02.003ISI: 000292665200003OAI: oai:DiVA.org:uu-156682DiVA: diva2:433371