uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Human cell lines used in a micro neutralization test for measuring influenza-neutralizing antibodies
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences.
Show others and affiliations
2006 (English)In: Scandinavian Journal of Immunology, ISSN 0300-9475, E-ISSN 1365-3083, Vol. 63, no 4, 257-263 p.Article in journal (Refereed) Published
Abstract [en]

An in situ neutralization test (NT) including ELISA for the measurement of influenza antigen was developed and evaluated. Two human cell lines, fibroblasts (HS27) cells and salivary gland epithelial duct (HSG) cells, were compared with Madin-Darby Canine Kidney (MDCK) cells. The viral production in the human cell lines was lower than that for MDCK cells, which influenced the results of the assay in the HSG and HS27 cells. However, when lowering the infectious dose, the NT using HS27 cells gave a sensitive and stable assay with low background in the ELISA. The NT titres were very low when using HSG cells compared to MDCK cells. The HS27 NT was used to analyze the humoral response after an influenza A infection in patients from a placebo-controlled zanamivir study. We found no differences in NT titres between patients treated with zanamivir or placebo. The MDCK and HS27 NT gave higher titres and more pronounced titre differences than the gold standard haemagglutinin inhibition (HAI) assay. Compared to the HAI assay, the sensitive NT using HS27 cells also revealed heterologous NT-titre rises after influenza infection in the patients.

Place, publisher, year, edition, pages
2006. Vol. 63, no 4, 257-263 p.
Keyword [en]
Adolescent, Adult, Animals, Antibodies; Viral/*blood, Cell Line, Cell Membrane/metabolism, Dogs, Dose-Response Relationship; Immunologic, Double-Blind Method, Enzyme-Linked Immunosorbent Assay/*methods, Guanidines/pharmacology, Humans, Influenza A virus/*immunology/isolation & purification, Microscopy; Electron, Middle Aged, N-Acetylneuraminic Acid/analysis, Neutralization Tests/*methods, Pyrans/pharmacology, Sialic Acids/pharmacology, Zanamivir
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-15655DOI: 10.1111/j.1365-3083.2006.01740.xPubMedID: 16623925OAI: oai:DiVA.org:uu-15655DiVA: diva2:43426
Available from: 2008-02-29 Created: 2008-02-29 Last updated: 2010-10-14Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMedhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=16623925&dopt=Citation

Search in DiVA

By author/editor
Pauksens, Karlis
By organisation
Department of Medical Sciences
In the same journal
Scandinavian Journal of Immunology
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 152 hits
ReferencesLink to record
Permanent link

Direct link