uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
An on-pathway intermediate in the folding of a PDZ domain
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Show others and affiliations
2007 (English)In: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 282, no 12, 8568-8572 p.Article in journal (Refereed) Published
Abstract [en]

The folding pathways of some proteins include the population of partially structured species en route to the native state. Identification and characterization of these folding intermediates are particularly difficult as they are often only transiently populated and play different mechanistic roles, being either on-pathway productive species or off-pathway kinetic traps. To define the role of folding intermediates, a quantitative analysis of the folding and unfolding rate constants over a wide range of denaturant concentration is often required. Such a task is further complicated by the reversible nature of the folding reaction, which implies the observed kinetics to be governed by a complex combination of different microscopic rate constants. Here, we tackled this problem by measuring directly the folding rate constant under highly denaturing conditions, namely by inducing the folding of a PDZ domain through a quasi-irreversible binding reaction with a specific peptide. In analogy with previous works based on hydrogen exchange experiments, we present evidence that the folding pathway of the PDZ domain involves the formation of an obligatory on-pathway intermediate. The results presented exemplify a novel type of kinetic test to detect an on-pathway folding intermediate.

Place, publisher, year, edition, pages
2007. Vol. 282, no 12, 8568-8572 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-15796DOI: 10.1074/jbc.M611026200ISI: 000245780300002PubMedID: 17251182OAI: oai:DiVA.org:uu-15796DiVA: diva2:43567
Available from: 2008-03-06 Created: 2008-03-06 Last updated: 2013-07-09Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Ivarsson, YlvaJemth, Per
By organisation
Department of Medical Biochemistry and Microbiology
In the same journal
Journal of Biological Chemistry
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 497 hits
ReferencesLink to record
Permanent link

Direct link