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Dose-response relationship for alpha-tocopherol prevention of ultraviolet radiation induced cataract in rat
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Ophthalmology. (Per Söderberg)
Inst. för Klinisk Neurovetenskap, KI. (Per Söderberg)
Inst. för Klinisk Neurovetenskap, KI. (Per Söderberg)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Ophthalmology. (Per Söderberg)
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2011 (English)In: Experimental Eye Research, ISSN 0014-4835, E-ISSN 1096-0007, Vol. 93, no 1, 91-97 p.Article in journal (Refereed) Published
Abstract [en]

The purpose of this study is to establish the dose response relationship for alpha-tocopherol protection of ultraviolet radiation (UVR) induced cataract in the rat. Four groups of 20 six-week-old albino Sprague Dawley rats received 5, 25, 50, and 100 IU/day alpha-tocopherol, whilst another group of 20 rats without any alpha-tocopherol feeding was the control group. After 4 weeks of feeding, each rat was unilaterally exposed to 8 kJ/m(2) UVR-300 nm for 15 min. At 1 week after exposure, the rats were sacrificed and lens light scattering was measured quantitatively. Lens total reduced (GSH) and oxidized (GSSG) glutathione; glutathione reductase (GR) and peroxidase (GPx) were determined spectrophotometrically. The UVR-exposed lenses in the alpha-tocopherol fed groups developed superficial cataract, whereas lenses in the control group developed cortical and equatorial opacities. Light scattering in lenses from the alpha-tocopherol-supplemented rats was lower than in lenses from the control group. The difference of light scattering between the exposed and contralateral non-exposed lens decreased with increasing doses of alpha-tocopherol to an asymptote level. UVR-exposure caused a significant depletion of lens GSH in rats without or at low alpha-tocopherol supplementation. The depletion of GSH became less with higher alpha-tocopherol supplementation. There was no detectable difference in lens GSSG, GR or GPx at any level of alpha-tocopherol supplementation. Orally administered alpha-tocopherol dose dependently protects against UVR-induced cataract. The protection is associated with an alpha-tocopherol dose-dependent GSH depletion secondary to UVR exposure. UVR-induced light scattering only occurs if the GSH depletion exceeds a threshold.

Place, publisher, year, edition, pages
2011. Vol. 93, no 1, 91-97 p.
Keyword [en]
forward light scattering, lens, cataract, ultraviolet radiation, alpha-tocopherol, dose-dependent
National Category
Ophthalmology
Identifiers
URN: urn:nbn:se:uu:diva-156961DOI: 10.1016/j.exer.2011.05.002ISI: 000293042200011PubMedID: 21620831OAI: oai:DiVA.org:uu-156961DiVA: diva2:435822
Funder
Swedish Research Council, K2008-63X-15035-05-2
Available from: 2011-08-20 Created: 2011-08-11 Last updated: 2017-12-08Bibliographically approved

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