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Distribution of ribosomes in action and at rest in Escherichia coli: involvement of bacterial cytoskeleton
Uppsala University, Disciplinary Domain of Science and Technology, Faculty of Science and Technology.
Uppsala University, Disciplinary Domain of Science and Technology, Faculty of Science and Technology. (Department of Cell and Molecular Biology)
Uppsala University, Disciplinary Domain of Science and Technology, Faculty of Science and Technology.
Uppsala University, Disciplinary Domain of Science and Technology, Faculty of Science and Technology.
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(English)Manuscript (preprint) (Other academic)
Identifiers
URN: urn:nbn:se:uu:diva-157689OAI: oai:DiVA.org:uu-157689DiVA: diva2:436082
Available from: 2011-08-22 Created: 2011-08-22 Last updated: 2012-03-06
In thesis
1. Dynamic Organization of Molecular Machines in Bacteria
Open this publication in new window or tab >>Dynamic Organization of Molecular Machines in Bacteria
2011 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Bacterial cells were once treated as membrane-enclosed bags of cytoplasm: a homogeneous, undifferentiated suspension in which polymers (proteins, nucleic acids, etc.) and small molecules diffused freely to interact with each other. Biochemical studies have determined the molecular mechanisms underlying the biological processes of metabolism, replication and transcription-translation, etc. However, recent advancements in optical techniques armed with fluorescent tags for proteins and nucleic acids have increased our ability to peer into the interior of live bacterial cells. This has revealed an organized layout of multi-protein complexes, or molecular machines, dedicated to specific functions at defined sub-cellular locations; the timing of their assembly and/or rates of their activity being determined by available nutrition and environmental signals from the niche occupied by the organism.

In the present study, we have attempted to identify the intracellular location and organization of the molecular machines assembled for protein synthesis (ribosomes), DNA replication (replisomes) and cell division (divisome) in different bacteria. We have used the model system Escherichia coli as well as Helicobacter pylori and mycobacterial strains (Mycobacterium marinum and Mycobacterium smegmatis), which grow at different rates and move to dormancy late into stationary phase

Bacterial nucleoid plays a major role in organizing the location and movement of active ribosomes, replisomes and placement of divisome. While the active ribosomes appear to follow the dynamic folds of the bacterial nucleoid during cell growth in E. coli, inactive ribosomes appear to accumulate near the periphery. The replisome in H. pylori was visualized as a sharp, single focus upon SSB and DnaB co-localization in growing helical rods but disassembled into diffused fluorescence when the cells attained non-replicative coccoid stage. Our investigation into mycobacterial life-cycle revealed unique features such as an absence of a dedicated mid-cell site for divisome assembly and endosporulation upon entry into stationary phase.

In brief, we present the cell cycle-dependent subcellular organization of molecular machines in bacteria. 

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2011. 48 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 847
Keyword
molecular machines, bacteria, cell cycle, ribosome, replisome, divisome, spore, E. coli, H. pylori, Mycobacteria
National Category
Microbiology
Research subject
Microbiology
Identifiers
urn:nbn:se:uu:diva-157691 (URN)978-91-554-8140-7 (ISBN)
Public defence
2011-10-14, Room B41, Biomedicinskt centrum (BMC), Husargatan 3, Uppsala, 13:15 (English)
Opponent
Supervisors
Available from: 2011-09-23 Created: 2011-08-22 Last updated: 2013-03-14Bibliographically approved

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